D'Amico Ramona, Cordaro Marika, Siracusa Rosalba, Impellizzeri Daniela, Trovato Salinaro Angela, Scuto Maria, Ontario Maria Laura, Crea Roberto, Cuzzocrea Salvatore, Di Paola Rosanna, Fusco Roberta, Calabrese Vittorio
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy.
Department of Biomedical, Dental and Morphological and Functional Imaging, University of Messina, 98125 Messina, Italy.
Biomedicines. 2021 Nov 13;9(11):1683. doi: 10.3390/biomedicines9111683.
Fibromyalgia (FM) is a chronic condition characterized by persistent widespread pain that negatively affects the quality of life of patients. The WNT/β-catenin signaling pathway seems to be involved in central sensitization and different pain states. The objective of this study was to investigate the beneficial effects of a new compound called Hidrox (HD), containing 40-50% hydroxytyrosol, in counteracting the pain associated with FM. An FM-like model was induced in rats by subcutaneous injections of reserpine (1 mg/kg) for three consecutive days. Later, HD (10 mg/kg) was administered orally to the animals for seven days. Reserpine injections induced WNT/β-catenin pathway activation, release of pro-inflammatory mediators as well as a significant increase in oxidative stress. Daily treatment with HD was able to modulate the WNT/β-catenin and Nrf2 pathways and consequently attenuate the behavioral deficits and microglia activation induced by reserpine injection. These results indicate that nutritional consumption of HD can be considered as a new therapeutic approach for human FM.
纤维肌痛（FM）是一种慢性疾病，其特征为持续的广泛性疼痛，会对患者的生活质量产生负面影响。WNT/β-连环蛋白信号通路似乎参与了中枢敏化和不同的疼痛状态。本研究的目的是调查一种名为希德罗斯（HD）的新化合物（含有40-50%的羟基酪醇）在对抗与纤维肌痛相关疼痛方面的有益作用。通过连续三天皮下注射利血平（1毫克/千克）在大鼠中诱导出类似纤维肌痛的模型。随后，给动物口服HD（10毫克/千克），持续七天。利血平注射诱导了WNT/β-连环蛋白通路的激活、促炎介质的释放以及氧化应激的显著增加。每日用HD治疗能够调节WNT/β-连环蛋白和Nrf2通路，从而减轻利血平注射诱导的行为缺陷和小胶质细胞激活。这些结果表明，HD的营养摄入可被视为治疗人类纤维肌痛的一种新的治疗方法。
