Department of Poultry Diseases, National Veterinary Research Institute, Pulawy, Poland.
PLoS One. 2020 Sep 29;15(9):e0234532. doi: 10.1371/journal.pone.0234532. eCollection 2020.
This article describes the isolation, molecular characterization, and genotyping of two fowl adenovirus (FAdVs) strains with GenBank Accession numbers (MT478054, JSN-G033-18-L and MT478055, JSN-G033-18-B) obtained from the internal organs of black grouse (Lyrurus tetrix). This study also reveals the first confirmation of fowl adenovirus in Poland, supporting one of the hypotheses about the probability of fowl adenovirus interspecies transmission. The adenovirus strain sequences were investigated via phylogenetic analysis and were found to have an overall mean pairwise distance of 2.189. The heterogeneity, Relative Synonymous Codon Usage (RSCU), codon composition, and nucleotide frequencies were examined. Statistical analyses and Tajima's test for the examined sequences were carried out. The Maximum Likelihood for the examined sequences substitutions was performed. The results of the sequence analysis identified MT478054, JSN-G033-18-L and MT478055, JSN-G033-18-B as strains of fowl adenovirus 2/11/D, with the Fowl adenovirus D complete sequence showing a 93% match. Wild birds may act as a natural reservoir for FAdVs and likely play an important role in the spreading of these viruses in the environment. The findings reported here suggest horizontal transmission within and between avian species.
本文描述了从黑琴鸡（Lyrurus tetrix）内脏中分离到的两株禽腺病毒（FAdVs）的分离、分子特征和基因分型，它们的 GenBank 登录号分别为 MT478054（JSN-G033-18-L）和 MT478055（JSN-G033-18-B）。这项研究还首次证实了波兰存在禽腺病毒，支持了禽腺病毒种间传播的可能性假说之一。通过系统进化分析研究了腺病毒株序列，发现它们的总体平均成对距离为 2.189。研究还检查了异质性、相对同义密码子使用（RSCU）、密码子组成和核苷酸频率。对所研究的序列进行了统计分析和 Tajima 检验。对所研究的序列替换进行了最大似然分析。序列分析的结果表明，MT478054、JSN-G033-18-L 和 MT478055、JSN-G033-18-B 是禽腺病毒 2/11/D 株，禽腺病毒 D 完整序列的匹配率为 93%。野生鸟类可能是禽腺病毒的天然宿主，并可能在这些病毒在环境中的传播中发挥重要作用。这里报告的发现表明，禽腺病毒在禽种内和种间存在水平传播。
