SARS-CoV-2 于眼内环境中的出现。
Occurrence of SARS-CoV-2 in the intraocular milieu.
机构信息
Department of Ophthalmology, Medical University of Graz Auenbruggerplatz 4, 8036, Graz, Austria.
Diagnostic and Research Institute of Pathology, Medical University of Graz Neue Stiftingtalstraße 6, 8010, Graz, Austria.
出版信息
Exp Eye Res. 2020 Dec;201:108273. doi: 10.1016/j.exer.2020.108273. Epub 2020 Sep 28.
Abstract
The purpose of this research is to study the intraocular occurrence of SARS-CoV-2. In postmortem examinations, aqueous humor and the vitreous samples were collected. All individuals were previously positive in nasopharyngeal swabbing and cause of death was respiratory failure due to SARS-CoV-2 infection. Testing was done using quantitative RT-PCR. We included 16 aqueous humor and 16 vitreous samples for PCR testing. None of the results was positive for SARS-CoV-2. Human GAPDH genes to verify the presence of RNA was present in all aqueous humor samples (16/16, 100%) and 15/16 (93.8%) vitreous samples. In conclusion, this case series found no evidence of SARS-CoV-2 in the intraocular milieu.
摘要
本研究旨在研究 SARS-CoV-2 在眼内的发生情况。在尸检中，收集了房水和玻璃体样本。所有个体的鼻咽拭子先前均为阳性，死亡原因是 SARS-CoV-2 感染引起的呼吸衰竭。检测使用定量 RT-PCR 进行。我们纳入了 16 份房水和 16 份玻璃体样本进行 PCR 检测。SARS-CoV-2 的检测结果均为阴性。所有房水样本（16/16，100%）和 15/16（93.8%）玻璃体样本中均存在人 GAPDH 基因以验证 RNA 的存在。总之，本病例系列研究未发现眼内环境中有 SARS-CoV-2 的证据。
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