Hoffmann Angeline, Müller Thomas, Fingerle Volker, Silaghi Cornelia, Noll Matthias
Institute for Bioanalysis, Department of Applied Sciences, Coburg University of Applied Sciences and Arts, 96450 Coburg, Germany.
Synlab Medical Care Unit, Department of Molecular biology, Tick Laboratory, 92637 Weiden in der Oberpfalz, Germany.
Microorganisms. 2023 Jan 7;11(1):157. doi: 10.3390/microorganisms11010157.
The prevalence of potential human pathogenic members of the order Rickettsiales differs between Borrelia burgdorferi sensu lato-positive and -negative tick microbiomes. Here, co-infection of members of the order Rickettsiales, such as Rickettsia spp., Anaplasma phagocytophilum, Wolbachia pipientis, and Neoehrlichia mikurensis as well as B. burgdorferi s.l. in the tick microbiome was addressed. This study used conventional PCRs to investigate the diversity and prevalence of the before-mentioned bacteria in 760 nucleic acid extracts of I. ricinus ticks detached from humans, which were previously tested for B. burgdorferi s.l.. A gltA gene-based amplicon sequencing approach was performed to identify Rickettsia species. The prevalence of Rickettsia spp. (16.7%, n = 127) and W. pipientis (15.9%, n = 121) were similar, while A. phagocytophilum was found in 2.8% (n = 21) and N. mikurensis in 0.1% (n = 1) of all ticks. Co-infection of B. burgdorferi s. l. with Rickettsia spp. was most frequent. The gltA gene sequencing indicated that Rickettsia helvetica was the dominant Rickettsia species in tick microbiomes. Moreover, R, monacensis and R. raoultii were correlated with autumn and area south, respectively, and a negative B. burgdorferi s. l. finding. Almost every fifth tick carried DNA of at least two of the human pathogenic bacteria studied here.
立克次氏体目中潜在的人类致病成员的流行情况在狭义伯氏疏螺旋体阳性和阴性蜱微生物群之间存在差异。在此，研究了立克次氏体目成员，如立克次氏体属、嗜吞噬细胞无形体、嗜皮菌沃尔巴克氏体和米库埃里新埃里希氏体以及狭义伯氏疏螺旋体在蜱微生物群中的共感染情况。本研究使用常规PCR来调查从人类身上采集的760份蓖麻硬蜱核酸提取物中上述细菌的多样性和流行情况，这些提取物之前已检测过狭义伯氏疏螺旋体。采用基于gltA基因的扩增子测序方法来鉴定立克次氏体物种。立克次氏体属（16.7%，n = 127）和嗜皮菌沃尔巴克氏体（15.9%，n = 121）的流行率相似，而在所有蜱中，嗜吞噬细胞无形体的检出率为2.8%（n = 21），米库埃里新埃里希氏体的检出率为0.1%（n = 1）。狭义伯氏疏螺旋体与立克次氏体属的共感染最为常见。gltA基因测序表明，瑞士立克次氏体是蜱微生物群中占主导地位的立克次氏体物种。此外，蒙纳西斯立克次氏体和拉乌尔蒂立克次氏体分别与秋季和南部地区相关，且与狭义伯氏疏螺旋体检测呈阴性相关。几乎每五只蜱中就有一只携带这里研究的至少两种人类致病细菌的DNA。
