Kollarcikova Miloslava, Faldynova Marcela, Matiasovicova Jitka, Jahodarova Eva, Kubasova Tereza, Seidlerova Zuzana, Babak Vladimir, Videnska Petra, Cizek Alois, Rychlik Ivan
Veterinary Research Institute, 62100 Brno, Czech Republic.
Faculty of Science, Masaryk's University, 62500 Brno, Czech Republic.
Microorganisms. 2020 Sep 27;8(10):1483. doi: 10.3390/microorganisms8101483.
Bacteroidaceae are common gut microbiota members in all warm-blooded animals. However, if Bacteroidaceae are to be used as probiotics, the species selected for different hosts should reflect the natural distribution. In this study, we therefore evaluated host adaptation of bacterial species belonging to the family Bacteroidaceae. , , , , and represented human-adapted species while , , , , , and were commonly detected in chicken but not human gut microbiota. There were 29 genes which were present in all human-adapted but absent from the genomes of all chicken isolates, and these included genes required for the pentose cycle and glutamate or histidine metabolism. These genes were expressed during an in vitro competitive assay, in which human-adapted species overgrew the chicken-adapted isolates. Not a single gene specific for the chicken-adapted species was found. Instead, chicken-adapted species exhibited signs of frequent horizontal gene transfer, of KUP, and genes in particular. The differences in host adaptation should be considered when the new generation of probiotics for humans or chickens is designed.
拟杆菌科是所有温血动物常见的肠道微生物群成员。然而，如果要将拟杆菌科用作益生菌，为不同宿主选择的物种应反映其自然分布。因此，在本研究中，我们评估了拟杆菌科细菌物种的宿主适应性。 、 、 、 、 代表适应人类的物种，而 、 、 、 、 、 通常在鸡的肠道微生物群中检测到，但在人类肠道微生物群中未检测到。有29个基因存在于所有适应人类的 中，但在所有鸡分离株的基因组中不存在，这些基因包括戊糖循环以及谷氨酸或组氨酸代谢所需的基因。这些基因在体外竞争试验中表达，在该试验中，适应人类的 物种数量超过了适应鸡的分离株。未发现任何特定于适应鸡的物种的基因。相反，适应鸡的物种表现出频繁水平基因转移的迹象，尤其是KUP、 和 基因。在设计新一代人类或鸡用益生菌时，应考虑宿主适应性的差异。
