T4 RNA连接酶反应中形成的中间体的合成与反应活性。
Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.
作者信息
Hoffmann P U, McLaughlin L W
出版信息
Nucleic Acids Res. 1987 Jul 10;15(13):5289-303. doi: 10.1093/nar/15.13.5289.
Abstract
The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be more efficient when the adenylated form of the donor molecule is used.
摘要
已经从由1-羟基苯并三唑活化的合适的磷酸化试剂制备了中间腺苷酸化供体衍生物A(5')pp(5')dTp和A(5')pp(5')GpGpGp。当使用供体分子的腺苷酸化形式时，由T4 RNA连接酶催化的供体和受体寡核苷酸之间的磷酸二酯键形成显示出更高的效率。
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