Schattenkerk C, Visser G M, van der Marel G A, van Boom J H
Nucleic Acids Res. 1983 Nov 11;11(21):7545-54. doi: 10.1093/nar/11.21.7545.
The bifunctional and crystalline phosphorylating agent morpholino-0,0-bis[1-benzotriazolyl]phosphate has been used for the preparation of a 3',5'-bis-phosphotriester intermediate of thymidine. The latter has been converted into ppTppp by the following consecutive steps; removal of the benzotriazolyl group followed by the addition of phosphoric acid and removal of the 2-(4-nitrophenyl)-ethyl group followed by the addition of pyrophosphoric acid.
双功能结晶性磷酸化试剂吗啉代-0,0-双[1-苯并三唑基]磷酸酯已用于制备胸腺嘧啶核苷的3',5'-双磷酸三酯中间体。后者通过以下连续步骤转化为ppTppp：去除苯并三唑基，随后添加磷酸，再去除2-(4-硝基苯基)乙基，接着添加焦磷酸。
