使用RNA连接酶合成寡脱氧核糖核苷酸。
The synthesis of oligodeoxyribonucleotides using RNA ligase.
作者信息
Hinton D M, Gumport R I
出版信息
Nucleic Acids Res. 1979 Sep 25;7(2):453-64. doi: 10.1093/nar/7.2.453.
Abstract
T4 RNA ligase catalyzes the addition of a single deoxyribonucleoside 3',5'-bisphosphate to the 3'-hydroxyl of oligodeoxyribonucleotides (Hinton et al. (1978) Biochemistry 17, 5091). We have determined improved conditions for this reaction which give yields equal to or greater than 85% when any of five common deoxyribonucleoside bisphosphate (pdAp, pdCp, pdGp, pdTp, or pdUp) are added to dA(PDA)4. A low ATP concentration, which is constantly maintained by a regeneration system composed of phosphocreatine, creatine kinase, and myokinase, contributes to the attainment of high yields. The addition of RNase A and spermine also enhances the rates and yields of the reactions. These conditions facilitate the use of RNA ligase as a reagent for the stepwise synthesis of DNA of defined sequence.
摘要
T4 RNA连接酶催化将单个脱氧核糖核苷3',5'-二磷酸添加到寡脱氧核糖核苷酸的3'-羟基上（辛顿等人，（1978年）《生物化学》17卷，5091页）。我们已经确定了该反应的改进条件，当将五种常见的脱氧核糖核苷二磷酸（pdAp、pdCp、pdGp、pdTp或pdUp）中的任何一种添加到dA(PDA)4中时，产率等于或大于85%。由磷酸肌酸、肌酸激酶和肌激酶组成的再生系统持续维持的低ATP浓度有助于实现高产率。添加核糖核酸酶A和精胺也能提高反应速率和产率。这些条件便于将RNA连接酶用作逐步合成特定序列DNA的试剂。
相似文献
1
The synthesis of oligodeoxyribonucleotides using RNA ligase.使用RNA连接酶合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1979 Sep 25;7(2):453-64. doi: 10.1093/nar/7.2.453.
2
The preparative synthesis of oligodeoxyribonucleotides using RNA ligase.使用RNA连接酶进行寡脱氧核糖核苷酸的制备合成。
Nucleic Acids Res. 1982 Mar 25;10(6):1877-94. doi: 10.1093/nar/10.6.1877.
3
T4 ribonucleic acid ligase joins single-strand oligo(deoxyribonucleotides).T4核糖核酸连接酶连接单链寡（脱氧核糖核苷酸）。
Biochemistry. 1980 Feb 19;19(4):635-42. doi: 10.1021/bi00545a005.
4
Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.T4 RNA连接酶反应中形成的中间体的合成与反应活性。
Nucleic Acids Res. 1987 Jul 10;15(13):5289-303. doi: 10.1093/nar/15.13.5289.
5
T4 RNA ligase as a nucleic acid synthesis and modification reagent.T4 RNA连接酶作为一种核酸合成与修饰试剂。
Nucleic Acids Symp Ser. 1980(7):167-71.
6
T4 RNA Ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides.T4 RNA连接酶将2'-脱氧核糖核苷3',5'-二磷酸连接到寡脱氧核糖核苷酸上。
Biochemistry. 1978 Nov 28;17(24):5091-7. doi: 10.1021/bi00617a004.
7
Using T4 RNA ligase with DNA substrates.将T4 RNA连接酶用于DNA底物。
Methods Enzymol. 1983;100:38-52. doi: 10.1016/0076-6879(83)00044-0.
8
Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.使用T4 RNA连接酶对tRNA和寡脱氧核苷酸进行荧光标记。
Nucleic Acids Res. 1984 Feb 24;12(4):1791-810. doi: 10.1093/nar/12.4.1791.
9
T4 RNA ligase catalyzed synthesis of base analogue-containing oligodeoxyribonucleotides and a characterization of their thermal stabilities.T4 RNA连接酶催化含碱基类似物的寡脱氧核糖核苷酸的合成及其热稳定性的表征。
Nucleic Acids Res. 1985 Dec 20;13(24):8665-84. doi: 10.1093/nar/13.24.8665.
10
Synthesis and purification of oligoribonucleotides using T4 RNA ligase and reverse-phase chromatography.使用T4 RNA连接酶和反相色谱法合成及纯化寡核糖核苷酸
Anal Biochem. 1985 Jan;144(1):110-7. doi: 10.1016/0003-2697(85)90091-0.
引用本文的文献
1
Recent progress in DNA data storage based on high-throughput DNA synthesis.基于高通量DNA合成的DNA数据存储的最新进展。
Biomed Eng Lett. 2024 May 3;14(5):993-1009. doi: 10.1007/s13534-024-00386-z. eCollection 2024 Sep.
2
Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.迷你综述：基于酶的DNA合成及用于数据存储的选择性检索
Comput Struct Biotechnol J. 2021 Apr 25;19:2468-2476. doi: 10.1016/j.csbj.2021.04.057. eCollection 2021.
3
Practical and general synthesis of 5'-adenylated RNA (5'-AppRNA).5'-腺苷酸化RNA（5'-AppRNA）的实用通用合成方法。
RNA. 2004 Apr;10(4):731-46. doi: 10.1261/rna.5247704.
4
Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.利用RNA连接酶，以活化的单核苷酸及其类似物在3'方向上延伸寡核苷酸。
Nucleic Acids Res. 1980 Feb 11;8(3):601-10. doi: 10.1093/nar/8.3.601.
5
The preparative synthesis of oligodeoxyribonucleotides using RNA ligase.使用RNA连接酶进行寡脱氧核糖核苷酸的制备合成。
Nucleic Acids Res. 1982 Mar 25;10(6):1877-94. doi: 10.1093/nar/10.6.1877.
6
Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase.使用T4 RNA连接酶对tRNA和寡脱氧核苷酸进行荧光标记。
Nucleic Acids Res. 1984 Feb 24;12(4):1791-810. doi: 10.1093/nar/12.4.1791.
7
Biotin and fluorescent labeling of RNA using T4 RNA ligase.使用T4 RNA连接酶对RNA进行生物素和荧光标记。
Nucleic Acids Res. 1983 Sep 24;11(18):6167-84. doi: 10.1093/nar/11.18.6167.
8
T4 RNA ligase catalyzed synthesis of base analogue-containing oligodeoxyribonucleotides and a characterization of their thermal stabilities.T4 RNA连接酶催化含碱基类似物的寡脱氧核糖核苷酸的合成及其热稳定性的表征。
Nucleic Acids Res. 1985 Dec 20;13(24):8665-84. doi: 10.1093/nar/13.24.8665.
9
Specific antiviral activity of a poly(L-lysine)-conjugated oligodeoxyribonucleotide sequence complementary to vesicular stomatitis virus N protein mRNA initiation site.一种与水疱性口炎病毒N蛋白mRNA起始位点互补的聚（L-赖氨酸）缀合寡脱氧核糖核苷酸序列的特异性抗病毒活性。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):648-52. doi: 10.1073/pnas.84.3.648.
10
Ligation with T4 RNA ligase of an oligodeoxyribonucleotide to covalently-linked cross-sectional base-pair analogues of short, normal, and long dimensions.用T4 RNA连接酶将寡脱氧核糖核苷酸连接到短、正常和长尺寸的共价连接的横截面碱基对类似物上。
Nucleic Acids Res. 1991 Feb 11;19(3):585-90. doi: 10.1093/nar/19.3.585.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Some effects of charge and structure upon ionic interactions of nucleic acids.电荷与结构对核酸离子相互作用的一些影响。
Biochim Biophys Acta. 1961 Jul 22;51:19-32. doi: 10.1016/0006-3002(61)91012-5.
3
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
4
Thermodynamics of the denaturation of lysozyme by guanidine hydrochloride. I. Depdendence on pH at 25 degrees.盐酸胍使溶菌酶变性的热力学。I. 25摄氏度下对pH值的依赖性
Biochemistry. 1969 Nov;8(11):4579-85. doi: 10.1021/bi00839a052.
5
Studies on ribonucleic acid ligase. Characterization of an adenosine triphosphate-inorganic pyrophosphate exchange reaction and demonstration of an enzyme-adenylate complex with T4 bacteriophage-induced enzyme.核糖核酸连接酶的研究。三磷酸腺苷-无机焦磷酸交换反应的特性以及与T4噬菌体诱导酶形成的酶-腺苷酸复合物的证明。
J Biol Chem. 1974 Dec 10;249(23):7447-56.
6
Purification and properties of bacteriophage T4-induced RNA ligase.噬菌体T4诱导的RNA连接酶的纯化及性质
Proc Natl Acad Sci U S A. 1972 Oct;69(10):3009-13. doi: 10.1073/pnas.69.10.3009.
7
T4-induced RNA ligase joins single-stranded oligoribonucleotides.T4 诱导的 RNA 连接酶连接单链寡核糖核苷酸。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):122-6. doi: 10.1073/pnas.72.1.122.
8
The use of terminal blocking groups for the specific joining of oligonucleotides in RNA ligase reactions containing equimolar concentrations of acceptor and donor molecules.在含有等摩尔浓度受体和供体分子的RNA连接酶反应中，使用末端封闭基团实现寡核苷酸的特异性连接。
Nucleic Acids Res. 1976 Nov;3(11):3157-66. doi: 10.1093/nar/3.11.3157.
9
Primed and unprimed synthesis of poly (dA-dT) by calf thymus DNA polymerase alpha.小牛胸腺DNA聚合酶α引发和未引发的聚（dA-dT）合成
J Biol Chem. 1977 Mar 25;252(6):1932-7.
10
T4 RNA Ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides.T4 RNA连接酶将2'-脱氧核糖核苷3',5'-二磷酸连接到寡脱氧核糖核苷酸上。
Biochemistry. 1978 Nov 28;17(24):5091-7. doi: 10.1021/bi00617a004.
Zotero 插件