Adachi Tetsuya, Boschetto Francesco, Miyamoto Nao, Yamamoto Toshiro, Marin Elia, Zhu Wenliang, Kanamura Narisato, Tahara Yoshiro, Akiyoshi Kazunari, Mazda Osam, Nishimura Ichiro, Pezzotti Giuseppe
Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.
Materials (Basel). 2020 Sep 25;13(19):4275. doi: 10.3390/ma13194275.
The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation-based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.
工程化三维（3D）骨移植替代物的移植是修复严重骨缺损的一种可行方法。对于大的骨缺损，即使有足够数量的细胞和细胞因子，也可能需要合适的3D支架来支持和刺激骨再生。在本研究中，我们评估了一种专门为骨组织工程开发的纳米凝胶构造3D支架——纳米凝胶交联无孔冻干（NanoCliP-FD）凝胶在体内的性能。通过微计算机断层扫描、拉曼光谱、组织学分析和基于同步辐射的傅里叶变换红外光谱等多种方法对样品进行了表征。NanoCliP-FD凝胶是先前开发的纳米凝胶交联多孔（NanoCliP）凝胶的改良版本，旨在实现骨矿化功能的高度改善。应用NanoCliP-FD凝胶后在体内生长的骨组织的光谱成像能够评估骨质量，并可用于判断NanoCliP-FD凝胶支架作为与大骨缺损相关的骨疾病治疗方式的可行性。
