新种及新种，从红海发现深海卤水 - 海水界面分离得到。
sp. nov. and sp. nov., Isolated from the Discovery Deep Brine-Seawater Interface in the Red Sea.
作者信息
Zhang Guishan, Dong Xiaoyan, Sun Yingjiao, Antunes André, Hikmawan Tyas, Haroon Mohamed Fauzi, Wang Junru, Stingl Ulrich
机构信息
Red Sea Research Center, King Abdullah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi Arabia.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
出版信息
Microorganisms. 2020 Sep 25;8(10):1475. doi: 10.3390/microorganisms8101475.
Two extremely halophilic archaeal strains, designated SB29 and SB3, were isolated from the brine-seawater interface of Discovery Deep in the Red Sea. Cells of both strains were pleomorphic (irregular polyhedrals, ovals, and rods) and stained Gram-negative; colonies were pigmented pink. The sequence similarity of the 16S rRNA gene of strain SB29 with that of its most closely related validly described species ( DSM 16227) and that of strain SB3 with its closest validly described relative ( ATCC 35960) was 98.1% and 98.6%, respectively. The incomplete draft genomes of SB29 and SB3 are 3,871,125 bp and 3,904,985 bp in size, respectively, and their DNA G + C contents are 60.75% and 65.64%, respectively. The highest ANI values between the genomes of SB29 and SB3 and the most closely related genomes in GenBank were determined as 82.6% ( ATCC BAA-897, GenBank accession no. GCA_000337835.1) and 92.6% ( ATCC 35960, GenBank accession no. GCA_000337795.1), respectively. These data indicate that the two new isolates cannot be classified into any recognized species of the genus , and, therefore, two novel species of the genus are proposed: sp. nov. (type strain SB29 = JCM 19567 = CGMCC 1.14960) and sp. nov. (type strain SB3 = JCM 19566 = CGMCC 1.14958).
从红海发现海渊的卤水 - 海水界面分离出两株极端嗜盐古菌菌株，分别命名为SB29和SB3。两株菌株的细胞均呈多形性（不规则多面体、椭圆形和杆状），革兰氏染色阴性；菌落呈粉红色。菌株SB29的16S rRNA基因与其最密切相关的有效描述种（DSM 16227）的序列相似性为98.1%，菌株SB3与其最密切相关的有效描述亲缘种（ATCC 35960）的序列相似性为98.6%。SB29和SB3的不完整基因组草图大小分别为3,871,125 bp和3,904,985 bp，其DNA G + C含量分别为60.75%和65.64%。SB29和SB3的基因组与GenBank中最密切相关基因组之间的最高ANI值分别确定为82.6%（ATCC BAA - 897，GenBank登录号GCA_000337835.1）和92.6%（ATCC 35960，GenBank登录号GCA_000337795.1）。这些数据表明这两株新分离株不能归入该属的任何已知种，因此，提出该属的两个新种： sp. nov.（模式菌株SB29 = JCM 19567 = CGMCC 1.14960）和 sp. nov.（模式菌株SB3 = JCM 19566 = CGMCC 1.14958）。
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