Zhang Guishan, Haroon Mohamed Fauzi, Zhang Ruifu, Dong Xiaoyan, Wang Dandan, Liu Yunpeng, Xun Weibing, Dong Xiuzhu, Stingl Ulrich
Red Sea Research Center, King Abdulah University of Science and Technology, Thuwal Jeddah 23955-6900, Saudi Arabia.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4624-4631. doi: 10.1099/ijsem.0.002344. Epub 2017 Oct 12.
Two moderately halophilic marine bacterial strains of the family Rhodobacteraceae, designated ZGT108 and ZGT118, were isolated from the brine-seawater interface at Erba Deep in the Red Sea (Saudi Arabia). Cells of both strains were aerobic, rod-shaped, non-motile, and Gram-stain-negative. The sequence similarity of the 16S rRNA genes of strains ZGT108 and ZGT118 was 94.9 %. The highest 16S rRNA gene sequence similarity of strain ZGT108 to its closest relative, Ruegeria conchae JCM 17315, was 98.9 %, while the 16S rRNA gene of ZGT118 was most closely related to that of Ruegeria intermedia LMG 25539 (97.7 % similarity). The sizes of the draft genomes as presented here are 4 258 055 bp (strain ZGT108) and 4 012 109 bp (strain ZGT118), and the G+C contents of the draft genomes are 56.68 mol% (ZGT108) and 62.94 mol% (ZGT108). The combined physiological, biochemical, phylogenetic and genotypic data supported placement of both strains in the genus Ruegeria and indicated that the two strains are distinct from each other as well as from all other members in the genus Ruegeria. This was also confirmed by low DNA-DNA hybridization values (<43.6 %) and low ANI values (<91.8 %) between both strains and the most closely related Ruegeria species. Therefore, we propose two novel species in the genus Ruegeria to accommodate these novel isolates: Ruegeriaprofundi sp. nov. (type strain ZGT108=JCM 19518=ACCC 19861) and Ruegeriamarisrubri sp. nov. (type strain ZGT118=JCM 19519=ACCC 19862).
从红海（沙特阿拉伯）埃尔巴深海的卤水 - 海水界面分离出两株红杆菌科的中度嗜盐海洋细菌菌株，分别命名为ZGT108和ZGT118。两株菌株的细胞均为需氧型、杆状、无运动性且革兰氏染色阴性。菌株ZGT108和ZGT118的16S rRNA基因序列相似性为94.9%。菌株ZGT108与其最接近的亲缘种康氏鲁杰氏菌JCM 17315的16S rRNA基因序列最高相似性为98.9%，而ZGT118的16S rRNA基因与中间鲁杰氏菌LMG 25539的最为相似（相似性为97.7%）。此处呈现的草图基因组大小分别为4258055 bp（菌株ZGT108）和4012109 bp（菌株ZGT118），草图基因组的G+C含量分别为56.68 mol%（ZGT108）和62.94 mol%（ZGT118）。综合的生理、生化、系统发育和基因型数据支持将这两株菌株归入鲁杰氏菌属，且表明这两株菌株彼此不同，也与鲁杰氏菌属的所有其他成员不同。两株菌株与最接近的鲁杰氏菌物种之间的低DNA - DNA杂交值（<43.6%）和低ANI值（<91.8%）也证实了这一点。因此，我们提议在鲁杰氏菌属中设立两个新物种以容纳这些新分离株：深海鲁杰氏菌（Ruegeria profundi sp. nov.）（模式菌株ZGT108 = JCM 19518 = ACCC 19861）和红海鲁杰氏菌（Ruegeria marisrubri sp. nov.）（模式菌株ZGT118 = JCM 19519 = ACCC 19862）。
