Bardwell J C, Craig E A
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5177-81. doi: 10.1073/pnas.84.15.5177.
We have isolated a gene from Escherichia coli homologous to the gene encoding the Mr 83,000 Drosophila heat shock protein (hsp83). In E. coli the protein homologous to hsp83 is a heat shock protein called C62.5. The predicted amino acid sequence of C62.5 is 41% and 42% identical to the Drosophila and human hsp83 proteins, respectively. Selected regions of the protein have conservation as high as 90%. The gene encoding C62.5 (named htpG) is located between the dnaZ and adk genes at 11.1 minutes on the E. coli chromosome. The htpG gene appears to be a newly identified locus. The isolation of an E. coli homologue of hsp83 illustrates the remarkable conservation of heat shock proteins in evolution and will facilitate genetic and biochemical experiments aimed at determining the function of hsp83.
我们从大肠杆菌中分离出了一个与编码83,000道尔顿果蝇热休克蛋白（hsp83）的基因同源的基因。在大肠杆菌中，与hsp83同源的蛋白质是一种名为C62.5的热休克蛋白。C62.5的预测氨基酸序列与果蝇和人类hsp83蛋白分别有41%和42%的同一性。该蛋白质的选定区域保守性高达90%。编码C62.5的基因（命名为htpG）位于大肠杆菌染色体上11.1分钟处的dnaZ和adk基因之间。htpG基因似乎是一个新发现的基因座。hsp83的大肠杆菌同源物的分离说明了热休克蛋白在进化过程中的显著保守性，并将有助于旨在确定hsp83功能的遗传和生化实验。
