Holmgren R, Corces V, Morimoto R, Blackman R, Meselson M
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3775-8. doi: 10.1073/pnas.78.6.3775.
We report nucleotide sequences of the regions surrounding the 5' ends of the genes for Drosophila melanogaster heat-shock proteins hsp83, hsp68, and hsp26, located at chromosome positions 63BC, 95D, and 67B, respectively. As in other eukaryotic genes, the sequence T-A-T-A-A-A-A-T occurs about 30 nucleotides upstream from the sites of mRNA initiation. Three additional sequence homologies and a dyad symmetry were noted at approximately corresponding locations in the three genes and in the gene for another heat-shock protein, hsp70. We also found an intron near the 5' end of the hsp83 gene, with sequences at its boundaries typical of splice sites found in other organisms.
我们报告了黑腹果蝇热休克蛋白hsp83、hsp68和hsp26基因5'端周围区域的核苷酸序列，它们分别位于染色体的63BC、95D和67B位置。与其他真核基因一样，序列T-A-T-A-A-A-A-T出现在mRNA起始位点上游约30个核苷酸处。在这三个基因以及另一个热休克蛋白hsp70基因的大致对应位置，还发现了另外三个序列同源性和一个二元对称结构。我们还在hsp83基因的5'端附近发现了一个内含子，其边界处的序列是其他生物体中典型的剪接位点序列。
