Xu Pingping, Feng Wenpo, Wang Mei, Zhang Ling, Liang Gaofeng, Jing Aihua
School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China.
Medical College, Pingdingshan University, Pingdingshan 467000, China.
Micromachines (Basel). 2021 Oct 16;12(10):1256. doi: 10.3390/mi12101256.
The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH-GO) were the label of secondary antibodies (Ab), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab/PdAuCu NPs/NH-GO catalyzed the reduction of HO in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL and a linear detection range from 0.1 pg mL to 200 ng mL. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.
肿瘤标志物的早期检测在癌症治疗中具有重要作用。在此，开发了一种新型的三明治型电化学免疫传感器，用于早期无标记检测癌症生物标志物癌胚抗原（CEA）。树枝状三叶扇状钯金铜纳米颗粒（PdAuCu NPs）/胺功能化氧化石墨烯（NH-GO）作为二抗（Ab）的标记物，金纳米颗粒修饰的聚多巴胺（Au/PDA）固定在丝网印刷碳电极（SPCE）上作为基底材料。树枝状三叶扇状PdAuCu NPs/NH-GO通过简单的水热法合成，并用于固定具有大表面积、增强催化性能和良好吸附性的抗体（Ab）以放大电流信号。随后，Ab/PdAuCu NPs/NH-GO在三明治型免疫反应中催化HO的还原。在最佳条件下，该免疫传感器对CEA表现出令人满意的响应，检测限为0.07 pg/mL，线性检测范围为0.1 pg/mL至200 ng/mL。所提出的免疫传感器足够适合CEA的实际样品分析，在癌症早期诊断中具有临床价值。
