Pharmaceutics Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Chemistry Department, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran.
Anal Chim Acta. 2020 Oct 9;1133:48-57. doi: 10.1016/j.aca.2020.07.071. Epub 2020 Aug 12.
The present research reported a new electrochemical biosensor based on ds-DNA/Eu doped NiO/CPE to detect amsacrine. Therefore, UV-Vis spectrophotometry, docking, and differential pulse voltammetry (DPV) have been used to study the interactions between amsacrine and dsDNA. Then, experimental parameters affected DNA immobilization and interactions between amsacrine and ds-DNA have been optimized. Afterwards, guanine oxidation peak current of ds-DNA has been chosen as a signal to analyze amsacrine in a concentration ranging between 0.1 and 100.0 μM and finally, limit of detection (LOD) of 0.05 μM has been calculated at optimal condition. Ultimately, it was found that the suggested biosensor is able to determine amsacrine in human serum and urine samples successfully.
本研究报道了一种基于 ds-DNA/Eu 掺杂 NiO/CPE 的新型电化学生物传感器，用于检测安吖啶。因此，采用紫外可见分光光度法、对接和差分脉冲伏安法（DPV）研究了安吖啶与 dsDNA 之间的相互作用。然后，优化了影响 DNA 固定化和安吖啶与 ds-DNA 之间相互作用的实验参数。之后，选择 ds-DNA 的鸟嘌呤氧化峰电流作为信号，在 0.1 至 100.0 μM 的浓度范围内分析安吖啶，并在最佳条件下计算出检测限（LOD）为 0.05 μM。最终，发现所提出的生物传感器能够成功地测定人血清和尿液样本中的安吖啶。
