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一种基于具有三叶草状表面的三维立方 Eu/CuO 纳米结构的无标记电化学生物传感器，用于测定抗癌药物阿糖胞苷。

A label-free electrochemical biosensor based on 3D cubic Eu/CuO nanostructures with clover-like faces for the determination of anticancer drug cytarabine.

作者信息

Foroughi Mohammad Mehdi, Jahani Shohreh, Aramesh-Broujeni Zahra, Rostaminasab Dolatabad Meisam

机构信息

Department of Chemistry, Islamic Azad University Kerman Branch Kerman Iran

Noncommunicable Diseases Research Center, Bam University of Medical Sciences Bam Iran.

出版信息

RSC Adv. 2021 May 13;11(28):17514-17525. doi: 10.1039/d1ra01372f. eCollection 2021 May 6.

DOI:10.1039/d1ra01372f
PMID:35479699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9033006/
Abstract

The present research utilized a simplified procedure for developing a novel electro-chemical DNA biosensor based on a carbon paste electrode (CPE) modified with three-dimensional (3D) cubic Eu/CuO nanostructures with clover-like faces (Eu/CuO CLFNs). The modified electrode was applied to monitor electro-chemical interactions between dsDNA and cytarabine for the first time. Then, the decreased oxidation signal of guanine following the interactions between cytarabine and dsDNA was utilized as an indicator for selectively determining cytarabine using differential pulse voltammetry (DPV). According to the findings, the oxidation peak current of guanine was linearly proportionate with the cytarabine concentration in the range between 0.01 and 90 μM. Additionally, the limit of quantification (LOQ) and the limit of detection (LOD) respectively equaled 9.4 nM and 2.8 nM. In addition, the repeatability, applicability and reproducibility of this analysis to drug dosage forms and human serum samples were investigated. Furthermore, UV-vis spectroscopy, DPV, docking and viscosity measurements were applied to elucidate the interaction mechanism of dsDNA with cytarabine. It was found that this DNA biosensor may be utilized to sensitively, accurately and rapidly determine cytarabine.

摘要

本研究采用了一种简化程序，用于开发一种基于用具有三叶草状面的三维（3D）立方Eu/CuO纳米结构（Eu/CuO CLFNs）修饰的碳糊电极（CPE）的新型电化学DNA生物传感器。首次将修饰电极用于监测双链DNA（dsDNA）与阿糖胞苷之间的电化学相互作用。然后，利用阿糖胞苷与dsDNA相互作用后鸟嘌呤氧化信号的降低作为指示剂，采用差分脉冲伏安法（DPV）选择性测定阿糖胞苷。根据研究结果，鸟嘌呤的氧化峰电流与阿糖胞苷浓度在0.01至90μM范围内呈线性比例关系。此外，定量限（LOQ）和检测限（LOD）分别为9.4 nM和2.8 nM。此外，还研究了该分析方法对药物剂型和人血清样品的重复性、适用性和重现性。此外，采用紫外可见光谱、DPV、对接和粘度测量等方法阐明dsDNA与阿糖胞苷的相互作用机制。结果发现，这种DNA生物传感器可用于灵敏、准确和快速地测定阿糖胞苷。

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