Amirfakhryan Elham, Davarnia Behzad, Jeddi Farhad, Najafzadeh Nowruz
Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Biochemistry, School of Medicine, Ardabil University of Medical University, Ardabil, Iran.
Avicenna J Phytomed. 2020 Sep-Oct;10(5):460-471.
Although azelaic acid is effective for treatment of acne and rosacea, the biological activity of azelaic acid and the effect of its combination therapy with minoxidil were not elucidated with regard to hair growth.
In this study, mouse vibrissae follicles were dissected on day 10 after depilation. Then, the bulb and bulge cells of the hair follicle were treated with minoxidil and azelaic acid for 10 days to evaluate Sonic hedgehog (Shh) protein expression. Moreover, bulge and bulb cells of the hair follicles were cultivated and the expression of , and mRNA levels was evaluated using real-time polymerase chain reaction (PCR) analysis. We further investigated the protective effects of azelaic acid against ultraviolet B (UVB) irradiation in cultured bulb and bulge cells by determining catalase activity. An irradiation dose of 20 mJ/cm UVB for 4 sec was chosen.
The results showed that catalase activity significantly (p<0.05) increased in the bulge cells after exposure to 2.5 mM and 25 mM azelaic acid. Meanwhile, treatment of the bulb cells with azelaic acid (2.5 and 25 mM) did not cause significant changes in catalase activity. We also found that azelaic acid (25 mM) alone upregulated and expression in the bulge cells and 100 µ minoxidil caused and overexpression in the bulb region of the hair follicle. Moreover, minoxidil (100 µM) alone and in combination with azelaic acid (25 mM) led to Shh protein overexpression in the hair follicles and in organ culture.
Our results indicated a potential role for azelaic acid in the protection of bulge cells from UVB damage and its combination with minoxidil may activate hair growth through overexpression of Shh protein.
尽管壬二酸对痤疮和酒渣鼻有效，但关于毛发生长，壬二酸的生物活性及其与米诺地尔联合治疗的效果尚未阐明。
在本研究中，脱毛后第10天解剖小鼠触须毛囊。然后，用米诺地尔和壬二酸处理毛囊的球部和隆突细胞10天，以评估音猬因子（Shh）蛋白表达。此外，培养毛囊的隆突和球部细胞，并使用实时聚合酶链反应（PCR）分析评估 、 和 mRNA水平的表达。我们通过测定过氧化氢酶活性，进一步研究了壬二酸对培养的球部和隆突细胞中紫外线B（UVB）照射的保护作用。选择20 mJ/cm 的UVB照射剂量，照射4秒。
结果显示，暴露于2.5 mM和25 mM壬二酸后，隆突细胞中的过氧化氢酶活性显著（p<0.05）增加。同时，用壬二酸（2.5和25 mM）处理球部细胞，过氧化氢酶活性没有显著变化。我们还发现，单独使用壬二酸（25 mM）可上调隆突细胞中的 和 表达，100 µ 米诺地尔可导致毛囊球部区域的 和 过表达。此外，单独使用米诺地尔（100 µM）以及与壬二酸（25 mM）联合使用，均可导致毛囊和器官培养物中Shh蛋白过表达。
我们的结果表明，壬二酸在保护隆突细胞免受UVB损伤方面具有潜在作用，其与米诺地尔联合使用可能通过Shh蛋白过表达激活毛发生长。
