Oaku Yoshihiro, Abe Akinari, Sasano Yohei, Sasaki Fuka, Kubota Chika, Yamamoto Naoki, Nagahama Tohru, Nagai Noriaki
Research & Development Headquarters Self-Medication, Taisho Pharmaceutical Co., Ltd., 1-403 Yoshinocho, Saitama 331-9530, Japan.
Faculty of Pharmacy, Kindai University, 3-4-1 Kowakae, Higashiosaka 577-8502, Japan.
Pharmaceutics. 2022 Apr 27;14(5):947. doi: 10.3390/pharmaceutics14050947.
We previously found that 1% minoxidil (MXD) nanoparticles prepared using a bead mill method led to an increase I n hair follicle delivery and hair growth in C57BL/6 mice. In the present study, we designed a nanoparticle formulation containing 5% MXD (MXD-NPs) using the bead mill method and investigated the hair-growth effect of MXD-NPs and a commercially available MXD solution (CA-MXD). Hair growth and in vivo permeation studies were conducted using C57BL/6 mice. Moreover, we examined the MXD contents in the upper (hair bulge) and the lower hair follicle (hair bulb) and observed the hair follicle epithelial stem cells (HFSC) by immunohistochemical staining using the CD200 antibody. The mean particle size of the MXD in the MXD-NPs was 139.8 nm ± 8.9 nm. The hair-growth effect of the MXD-NPs was higher than that of CA-MXD, and the MXD content in the hair bulge of mice treated with MXD-NPs was 7.4-fold that of the mice treated with CA-MXD. In addition, the activation of HFSC was observed around the bulge in the MXD-NPs-treated mice. We showed that MXD-NPs enable the accumulation of MXD in the upper hair follicles more efficiently than CA-MXD, leading the activation of HFSC and the hair growth.
我们之前发现，使用珠磨机法制备的1%米诺地尔（MXD）纳米颗粒可增加C57BL/6小鼠毛囊中的药物递送并促进毛发生长。在本研究中，我们使用珠磨机法设计了一种含有5% MXD的纳米颗粒制剂（MXD-NPs），并研究了MXD-NPs和市售MXD溶液（CA-MXD）的毛发生长效果。使用C57BL/6小鼠进行了毛发生长和体内渗透研究。此外，我们检测了毛囊上部（毛球）和下部（毛乳头）中的MXD含量，并使用CD200抗体通过免疫组织化学染色观察毛囊上皮干细胞（HFSC）。MXD-NPs中MXD的平均粒径为139.8 nm±8.9 nm。MXD-NPs的毛发生长效果高于CA-MXD，用MXD-NPs处理的小鼠毛球中的MXD含量是用CA-MXD处理的小鼠的7.4倍。此外，在MXD-NPs处理的小鼠的毛球周围观察到了HFSC的激活。我们表明，MXD-NPs比CA-MXD更有效地使MXD在上部毛囊中蓄积，从而导致HFSC的激活和毛发生长。
