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用于体外和体内研究的药物处理急性髓系白血病细胞与T细胞的共培养系统

Co-culture Systems of Drug-Treated Acute Myeloid Leukemia Cells and T Cells for In Vitro and In Vivo Study.

作者信息

Li Yangchan, Su Rui, Chen Jianjun

机构信息

Department of Systems Biology, Beckman Research Institute of City of Hope, Monrovia, CA 91016, USA.

Department of Radiation Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.

出版信息

STAR Protoc. 2020 Sep 18;1(2). doi: 10.1016/j.xpro.2020.100097. Epub 2020 Aug 28.

DOI:10.1016/j.xpro.2020.100097
PMID:32995754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7521668/
Abstract

A combination of immunotherapy and chemotherapy strategies could strengthen antitumor effects. This protocol elucidates a robust method via co-culturing drug pre-treated acute myeloid leukemia cells with CD3 T cells, derived from leukoreduction system chambers, for and study. We optimized several aspects of the procedures, including timing of drug treatment, quantification of tumor cells, and approach of combination of CD3 T cells with drug treatment . This enables the readouts of the interplay between drugs and T cells. For complete details on the use and execution of this protocol, please refer to Su et al. (2020).

摘要

免疫疗法和化疗策略相结合可以增强抗肿瘤效果。本方案阐明了一种通过将药物预处理的急性髓系白血病细胞与来自白细胞去除系统腔室的CD3 T细胞共培养进行[具体时间]和[具体研究内容]研究的可靠方法。我们优化了该程序的几个方面，包括药物治疗时间、肿瘤细胞定量以及CD3 T细胞与药物治疗的联合方式。这使得能够读出药物与T细胞之间的相互作用。有关本方案使用和执行的完整详细信息，请参考Su等人（2020年）的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/8606fd652cd7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/e7e89abb9304/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/d7bcff55e3fd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/aec017bd2ff8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/60455a479ebb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/8606fd652cd7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/e7e89abb9304/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/d7bcff55e3fd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/aec017bd2ff8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/60455a479ebb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3006/7580212/8606fd652cd7/gr4.jpg

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