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sp. nov.,分离自稻田水样。

sp. nov., isolated from water sampled from a rice paddy field.

机构信息

Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan ROC.

Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Nan-Tzu, Kaohsiung City 811, Taiwan ROC.

出版信息

Int J Syst Evol Microbiol. 2019 Dec;69(12):3843-3850. doi: 10.1099/ijsem.0.003693.

Abstract

Strain TER-1 was isolated from water sampled from a rice paddy field in Taiwan. Cells were Gram-negative, aerobic, motile, rod-shaped and formed pink-coloured colonies. Optimal growth occurred at 25-30 °C, pH 6-7 and in the presence of 0.5 % NaCl. Strain TER-1 could grow on C1 compounds such as methanol, formic acid, methylamine and dimethylamine as sole carbon source, and carry methanol dehydrogenase gene, which supports its methylotrophic metabolism. The results of phylogenetic analyses based on the 16S rRNA gene sequence, methanol dehydrogenase gene sequence and coding sequences of 92 protein clusters indicated that strain TER-1 formed a phylogenetic lineage in the genus . Strain TER-1 was most closely related to AR24 with 96.8 % 16S rRNA gene sequence similarity. Strain TER-1 showed 77.1-82.8 % average nucleotide identity and 16.4-20.2 % digital DNA-DNA hybridization identity with the strains of other species. The major fatty acid of strain TER-1 was Cω7. The predominant hydroxy fatty acid was C 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and two uncharacterized lipids. The only isoprenoid quinone was Q-10. The genomic DNA G+C content of strain TER-1 was 71.9 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TER-1 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TER-1 (=BCRC 81157=LMG 30931=KCTC 62864).

摘要

TER-1 菌株从台湾稻田水样中分离得到。细胞呈革兰氏阴性、需氧、能动、杆状,形成粉红色菌落。最佳生长条件为 25-30°C、pH 6-7 和 0.5%NaCl 存在。TER-1 菌株可以甲醇、甲酸、甲胺和二甲胺等 C1 化合物作为唯一碳源生长,并携带甲醇脱氢酶基因,支持其甲基营养代谢。基于 16S rRNA 基因序列、甲醇脱氢酶基因序列和 92 个蛋白质簇编码序列的系统发育分析结果表明,TER-1 菌株形成了一个在属中的系统发育分支。TER-1 菌株与 AR24 菌株的 16S rRNA 基因序列相似度最高,为 96.8%。TER-1 菌株与其他 种菌株的平均核苷酸同一性和数字 DNA-DNA 杂交同一性分别为 77.1-82.8%和 16.4-20.2%。TER-1 菌株的主要脂肪酸为 Cω7。主要羟基脂肪酸为 C3-OH。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱和两种未鉴定的脂类组成。唯一的异戊烯醌是 Q-10。TER-1 菌株的基因组 DNA G+C 含量为 71.9mol%。基于表型和基因型特征以及系统发育推断,TER-1 菌株应归类为属的一个新种,建议将其命名为 sp. nov.。模式菌株为 TER-1(=BCRC 81157=LMG 30931=KCTC 62864)。

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