LEFFAG-Laboratory of Physiopharmacology Study of Gastrointestinal Tract, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
Department of Pharmacology, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Inflammopharmacology. 2021 Feb;29(1):193-204. doi: 10.1007/s10787-020-00763-x. Epub 2020 Sep 29.
Euterpe oleracea Mart., commonly known as açaí, has been demonstrated to exhibit significantly antioxidant and inflammatory activities in experimental models. These effects of the hydroalcoholic extract from the açaí seed (ASE) were investigated in TNBS-induced (2,4,6-trinitrobenzenesulfonic acid) acute colitis model in rats. Wistar rats (180-220 g) were orally pretreated with saline (0.3 mL), ASE (10, 30 and 100 mg/kg) and dexamethasone (control group, 1 mg/kg) once daily for 3 days starting before TNBS instillation. On day 3 after TNBS, the animals were euthanized, the portion of distal colon was collected and washed with 0.9% saline for macroscopy and histological evaluation, glutathione (GSH) and malonyldialdehyde (MDA) levels, myeloperoxidase (MPO) and catalase (CAT) activity, nitrate and nitrite (NO/NO) concentration, pro-inflammatory cytokines levels and intestinal barrier integrity. We also evaluated Toll-like Receptor 4/cyclooxygenase-2/nuclear factor kappa B expression as a possible mechanism related to the ASE effects. Treatment with ASE 100 mg/kg decreased significantly macroscopic and microscopic damage induced by TNBS. In addition, MPO activity, TNF-α (tumor necrosis factor-alpha) and IL-1β (interleukin 1) levels were reduced in rats with colitis. ASE 100 mg/kg restored GSH and MDA levels, CAT activity, NO/NO concentration and improved the intestinal barrier integrity in the TNBS group. ASE 100 mg/kg significantly reduced TNBS-induced expression of the TLR4, COX-2 and NF-κB p65. ASE 100 mg/kg improved macroscopy and histological parameters, inflammation, intestinal barrier integrity and nitric and oxidative stress through the TLR-4/COX-2/NF-κB pathway.
大蕉，俗称巴西莓，已被证明在实验模型中具有显著的抗氧化和抗炎活性。本研究旨在探讨巴西莓籽油（ASE）对 2,4,6-三硝基苯磺酸（TNBS）诱导的大鼠急性结肠炎模型的影响。Wistar 大鼠（180-220 g）于 TNBS 灌注前 3 天每天经口给予生理盐水（0.3 mL）、ASE（10、30 和 100 mg/kg）和地塞米松（对照组，1 mg/kg）一次。在 TNBS 灌注后第 3 天，处死动物，收集远端结肠部分并以 0.9%生理盐水冲洗，进行大体观察和组织学评估、谷胱甘肽（GSH）和丙二醛（MDA）水平、髓过氧化物酶（MPO）和过氧化氢酶（CAT）活性、硝酸盐和亚硝酸盐（NO/NO）浓度、促炎细胞因子水平和肠道屏障完整性。我们还评估了 Toll 样受体 4/环氧化酶-2/核因子 kappa B 表达，作为与 ASE 作用相关的可能机制。ASE 100 mg/kg 治疗可显著减轻 TNBS 诱导的大体和显微镜下损伤。此外，MPO 活性、TNF-α（肿瘤坏死因子-α）和 IL-1β（白细胞介素 1）水平在结肠炎大鼠中降低。ASE 100 mg/kg 可恢复 GSH 和 MDA 水平、CAT 活性、NO/NO 浓度，并改善 TNBS 组的肠道屏障完整性。ASE 100 mg/kg 显著降低 TLR4、COX-2 和 NF-κB p65 的 TNBS 诱导表达。ASE 100 mg/kg 通过 TLR-4/COX-2/NF-κB 途径改善宏观和组织学参数、炎症、肠道屏障完整性和一氧化氮和氧化应激。
