Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.
International Environmental Research Institute, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
PLoS One. 2020 Sep 30;15(9):e0239457. doi: 10.1371/journal.pone.0239457. eCollection 2020.
This study aimed to detect Salmonella from retail meat collected from nine wet markets in Metro Manila, and identify the subtypes of Salmonella isolates using molecular serotyping assays from previously developed primers. Of the 720 collected meat samples, 57.64% were found to be Salmonella-contaminated. The most predominant serogroup was Salmonella O:3, and Salmonella serogroups O:4, O:6,7, O:8, O:9, and undetermined serogroups were also found. Most frequently detected isolates in bovine meat were S. 3:e,h:1,6 (putative identity: S. Anatum) and S: 4:e,h:1,2 (putative identity: S. Saintpaul), in porcine meat was S. 3:e,h:1,6 (putative identity: S. Anatum), and S. 8:i:z6 (putative identity: S. Kentucky) was common in poultry products. This study also demonstrated retail meat samples were contaminated with multiple Salmonella serogroups and serovars. This is the first Philippine study that utilized PCR-based assays to characterize Salmonella isolates down to a serovar level and provides baseline information regarding Salmonella prevalence and serovar distribution in retail meat. Molecular serotyping performed in this study can be used as an alternative approach to traditional serotyping in surveillance of Salmonella in the Philippines since the latter is expensive, time-consuming, and requires skilled technicians.
本研究旨在从马尼拉都会区的九个湿市场采集零售肉品样本，检测其中的沙门氏菌，并使用先前开发的引物进行分子血清型分析，鉴定沙门氏菌分离株的亚型。在采集的 720 个肉品样本中，有 57.64%被检出含有沙门氏菌。最主要的血清群是沙门氏菌 O:3，此外还发现了沙门氏菌血清群 O:4、O:6、7、O:8、O:9 和未确定的血清群。在牛肉类中最常检测到的分离株为 S. 3:e,h:1,6（假定身份：S. Anatum）和 S: 4:e,h:1,2（假定身份：S. Saintpaul），在猪肉类中为 S. 3:e,h:1,6（假定身份：S. Anatum），而在禽肉类中则为 S. 8:i:z6（假定身份：S. Kentucky）。本研究还表明，零售肉品样本受到多种沙门氏菌血清群和血清型的污染。这是菲律宾首例利用基于 PCR 的检测方法对沙门氏菌分离株进行血清型鉴定的研究，为零售肉品中沙门氏菌的流行情况和血清型分布提供了基线信息。本研究中进行的分子血清型鉴定可作为菲律宾沙门氏菌监测的传统血清型鉴定的替代方法，因为后者昂贵、耗时且需要技术熟练的技术人员。
