Institute of Biochemistry and Molecular Biology, College of Life and Health Sciences, Northeastern University, Shenyang, 110819, P. R. China.
Research Center of Tea and Tea Culture, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, P. R. China.
Mol Nutr Food Res. 2020 Nov;64(22):e2000353. doi: 10.1002/mnfr.202000353. Epub 2020 Oct 12.
Huangjinya is a light-sensitive tea mutant containing low levels of tea polyphenols. Currently, most studies focused on characteristics formation, free amino acid metabolism and phytochemical purification. The biological activity of Huangjinya black tea (HJBT) on metabolic syndrome regarding fecal metabolome modulation is unavailable and is studied herein.
High-fat diet (HFD)-fed mice are treated with HJBT for 9 weeks, various metabolic biomarkers and fecal metabolites are determined. HJBT reduces adipogenic and lipogenic gene expression, enhances lipolytic gene expression, decreases adipocyte expansion, and prevents the development of obesity. HJBT reduces lipogenic gene expression, increases fatty acid oxidation-related genes expression, which alleviates liver steatosis. HJBT enhances glucose/insulin tolerance, increases insulin/Akt signaling, attenuates hyperlipidemia and hyperglycemia, prevents the onset of insulin resistance. HJBT modulates bile acid metabolism, promotes secondary/primary bile acid ratio; increases short-chain fatty acids production, promotes saturated and polyunsaturated fatty acids content; reduces carnitines and phosphocholines, but increases myo-inositol content; decreases branched-chain and aromatic amino acids content; increases the metabolite content related to pentose phosphate pathway.
This study reported the association between fecal metabolome modulation and metabolism improvement due to HJBT administration, proposes HJBT as a dietary intervention for preventing obesity and metabolic disorders.
黄金甲是一种含有低水平茶多酚的光敏感茶突变体。目前，大多数研究都集中在特征形成、游离氨基酸代谢和植物化学物质纯化上。关于黄金甲黑茶（HJBT）对肠道代谢组调节代谢综合征的生物活性尚未可知，本文对此进行了研究。
用高脂肪饮食（HFD）喂养的小鼠用 HJBT 处理 9 周，测定各种代谢生物标志物和粪便代谢物。HJBT 降低了脂肪生成和脂肪生成基因的表达，增强了脂肪分解基因的表达，减少了脂肪细胞的扩张，并防止了肥胖的发展。HJBT 降低了脂肪生成基因的表达，增加了与脂肪酸氧化相关的基因表达，从而减轻了肝脂肪变性。HJBT 增强了葡萄糖/胰岛素耐量，增加了胰岛素/Akt 信号，减轻了高脂血症和高血糖，防止了胰岛素抵抗的发生。HJBT 调节胆汁酸代谢，促进次级/初级胆汁酸比值；增加短链脂肪酸的产生，促进饱和和多不饱和脂肪酸含量；降低肉碱和磷胆碱基，但增加肌醇含量；降低支链和芳香族氨基酸含量；增加与戊糖磷酸途径相关的代谢产物含量。
本研究报道了 HJBT 给药与粪便代谢组调节和代谢改善之间的关联，提出 HJBT 可作为预防肥胖和代谢紊乱的饮食干预措施。
