Ferraro Valentina Agnese, Carraro Silvia, Pirillo Paola, Gucciardi Antonina, Poloniato Gabriele, Stocchero Matteo, Giordano Giuseppe, Zanconato Stefania, Baraldi Eugenio
Department of Women's and Children's Health, University of Padova, 35128 Padova, Italy.
Institute of Pediatric Research (IRP), Fondazione Città della Speranza, 35128 Padova, Italy.
Metabolites. 2020 Sep 29;10(10):390. doi: 10.3390/metabo10100390.
"breathomics" enables indirect analysis of metabolic patterns underlying a respiratory disease. In this study, we analyze exhaled breath condensate (EBC) in asthmatic children before (T0) and after (T1) a three-week course of inhaled beclomethasone dipropionate (BDP).
we recruited steroid-naive asthmatic children for whom inhaled steroids were indicated and healthy children, evaluating asthma control, spirometry and EBC (in asthmatics at T0 and T1). A liquid-chromatography-mass-spectrometry untargeted analysis was applied to EBC and a mass spectrometry-based target analysis to urine samples.
metabolomic analysis discriminated asthmatic ( = 26) from healthy children ( = 16) at T0 and T1, discovering 108 and 65 features relevant for the discrimination, respectively. Searching metabolomics databases, seven putative biomarkers with a plausible role in asthma biochemical-metabolic processes were found. After BDP treatment, asthmatic children, in the face of an improved asthma control ( < 0.001) and lung function ( = 0.01), showed neither changes in EBC metabolomic profile nor in urinary endogenous steroid profile.
"breathomics" can discriminate asthmatic from healthy children, with prostaglandin, fatty acid and glycerophospholipid as putative markers. The three-week course of BDP-in spite of a significant clinical improvement-was not associated with changes in EBC metabolic arrangement and urinary steroid profile.
“呼吸代谢组学”能够间接分析呼吸系统疾病潜在的代谢模式。在本研究中，我们分析了哮喘儿童在吸入丙酸倍氯米松（BDP）为期三周的疗程之前（T0）和之后（T1）的呼出气冷凝液（EBC）。
我们招募了需要吸入类固醇治疗的初治哮喘儿童和健康儿童，评估哮喘控制情况、肺功能测定和EBC（哮喘儿童在T0和T1时）。对EBC进行液相色谱 - 质谱非靶向分析，对尿液样本进行基于质谱的靶向分析。
代谢组学分析在T0和T1时区分了哮喘儿童（n = 26）和健康儿童（n = 16），分别发现了108个和65个与区分相关的特征。在搜索代谢组学数据库时，发现了七种在哮喘生化代谢过程中可能起作用的假定生物标志物。BDP治疗后，哮喘儿童在哮喘控制改善（P < 0.001）和肺功能改善（P = 0.01）的情况下，EBC代谢组学谱和尿内源性类固醇谱均未发生变化。
“呼吸代谢组学”可以区分哮喘儿童和健康儿童，前列腺素、脂肪酸和甘油磷脂为假定标志物。尽管临床有显著改善，但为期三周的BDP疗程与EBC代谢排列和尿类固醇谱的变化无关。
