Wellkamp Marvin, García-Camacho Francisco, Durán-Riveroll Lorena M, Tebben Jan, Tillmann Urban, Krock Bernd
Alfred-Wegener-Institut für Polar und Meeresforschung, 27570 Bremerhaven, Germany.
Chemical Engineering Department, University of Almería, La Cañada, 04120 Almeria, Spain.
Mar Drugs. 2020 Sep 29;18(10):497. doi: 10.3390/md18100497.
Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.
扁藻毒素是由怀疑导致鱼类死亡的甲藻产生的聚酮化合物。在此，我们展示了一种用于鉴定和定量扁藻毒素（AM）的液相色谱 - 串联质谱（LC-MS/MS）方法。通过中性丢失（NL）扫描检测新型AM，然后通过选择反应监测（SRM）与已知AM一起进行定量。使用新方法，在八个分析菌株中的四个中检测到了AM，每个细胞中毒素含量最高为3680 fg。总共通过NL扫描检测到16种新型AM，并通过其碎片模式进行了表征。其中，两种物质是糖基化形式。这是首次检测到糖基化的AM。
