Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology, Getreidemarkt 9/163-164, 1060, Vienna, Austria.
Karl Chiari Lab for Orthopaedic Biology, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Sci Rep. 2020 Oct 1;10(1):16192. doi: 10.1038/s41598-020-73055-7.
Dermal fibroblast cell migration is a key process in a physiological wound healing. Therefore, the analysis of cell migration is crucial for wound healing research. In this study, lab-on-a-chip technology was used to investigate the effects of basic fibroblast growth factor (bFGF), mitomycin C (MMC), MEK1/2 inhibitor (U0126) and fetal calf serum (FCS) on human dermal fibroblast cell migration. The microdevice was fabricated consisting of microchannels, pneumatic lines and pneumatically-activated actuators by xurographic rapid prototyping. In contrast to current approaches in in vitro wound healing such as scratch assays and silicone inserts in wellplate format, which show high variability and poor reproducibility, the current system aims to automate the wounding procedure at high precision and reproducibility using lab-on-a-chip. Traumatic wounding was simulated on-chip on fibroblast cell monolayers by applying air pressure on the flexible circular membrane actuator. Wound closure was monitored using light microscopy and cell migration was evaluated using image analysis. The pneumatically controlled system generates highly reproducible wound sizes compared to the conventional wound healing assay. As proof-of-principle study wound healing was investigated in the presence of several stimulatory and inhibitory substances and culture including bFGF, MMC, U0126 MEK1/2 inhibitor as well as serum starvation to demonstrate the broad applicability of the proposed miniaturized culture microsystem.
皮肤成纤维细胞的迁移是生理伤口愈合的关键过程。因此，细胞迁移的分析对于伤口愈合研究至关重要。在这项研究中，我们使用微流控芯片技术研究了碱性成纤维细胞生长因子 (bFGF)、丝裂霉素 C (MMC)、MEK1/2 抑制剂 (U0126) 和胎牛血清 (FCS) 对人皮肤成纤维细胞迁移的影响。该微器件由微通道、气动线和气动激活执行器组成，通过 xurographic 快速原型制作而成。与目前在体外伤口愈合中使用的划痕测定法和微孔板格式的硅酮插入物等方法相比，当前系统旨在使用微流控芯片以高精度和可重复性自动进行创伤过程。通过在柔性圆形膜执行器上施加气压，在成纤维细胞单层上模拟芯片上的创伤性愈合。使用显微镜监测伤口闭合情况，并使用图像分析评估细胞迁移情况。与传统的伤口愈合测定法相比，气动控制的系统可产生高度可重复的伤口尺寸。作为原理验证研究，在存在几种刺激和抑制物质以及培养物的情况下研究了伤口愈合，包括 bFGF、MMC、U0126 MEK1/2 抑制剂以及血清饥饿，以证明所提出的小型化培养微系统的广泛适用性。
