越南两种变形翼病毒毒株的基因组分析及系统发育特征
Genome analysis and phylogenetic characterization of two deformed wing virus strains from in Vietnam.
作者信息
Thu Ha T, Lien Nguyen T K, Lanh Pham T, Duong Bui T T, Hoa Nguyen T, Phuoc Man H, Thai Pham H, Quyen Dong Van
机构信息
Laboratory of Molecular Microbiology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Laboratory of Functional Genomics, Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
出版信息
PeerJ. 2020 Sep 21;8:e9911. doi: 10.7717/peerj.9911. eCollection 2020.
BACKGROUND
Deformed wing virus (DWV) is a virulent virus that causes honeybee disease. DWV can exist as a latent infection in honeybees, outbreak into epidemics, and cause serious damage to beekeeping cross the world, including Vietnam.
METHODS
The two DWV strains circulating in Vietnamese honeybee, were first isolated from adult honeybees in North Vietnam (DWV-NVN) and South Vietnam (DWV-SVN). Their complete nucleotide sequences were determined, aligned, and compared with other DWV strains
RESULTS
The two Vietnamese DWV strains comprised 10,113 bp and contained a large single open reading frame (ORF) of 2,893 amino acids, initiating at nucleotide 1,130 and terminating at nucleotide 9,812. Multiple nucleotide sequence alignment between these two DWV-VN strains and DWV strains in was performed. The DWV-VN strains showed a low genetic identity (from 91.4% to 92.0%) with almost of these strains, but lower identities (89.2% and 89.4%) with UK2 and (89.6%) with the China2 strain. Low identities (91.7% and 91.9%) were also observed between the China3 strain (in ) and the DWV-VN strains, respectively. The deduced amino acid sequence alignment showed high genetic similarities (97.0%-97.9%) when the USA1, Chile, Italy1, France, UK1, UK2, Japan, Korea2, China1, China2 and China3 strains were compared to the DWV-VN strains. This ratio was 96.7% and 96.8% when the Korea1 strain was compared to the DWV-SVN and DWV-NVN strains, respectively. Numerous amino acid substitutions were identified in the L, VP3, and RdRp sequences. Notably, we observed six substitutions positioned at amino acids 27 (E > I), 98 (S > T), 120 (A > V), 153 (M > T), 170 (D > F), and 174 (Y > F) in the L protein, two amino acid changes at positions 980 (S > A) and 1032 (E > T) in VP3, and one amino acid change at position 2627 (R > C) unique to the DWV-VN strains. Phylogenetic analysis based on complete genome sequences, RdRp sequences and Simplot analysis indicated that there was a significant difference between DWV-VN strains in and DWV strains in . The results suggested that the genetic variations of the DWV-VN strains in help them to adapt geographical conditions and may lead to change the viral pathogenicity of DWV-VN strains.
背景
变形翅病毒（DWV）是一种导致蜜蜂患病的烈性病毒。DWV可在蜜蜂中以潜伏感染的形式存在，爆发成流行病，并对包括越南在内的全球养蜂业造成严重破坏。
方法
在越南蜜蜂中传播的两种DWV毒株，最初分别从越南北部（DWV-NVN）和南部（DWV-SVN）的成年蜜蜂中分离出来。测定了它们的完整核苷酸序列，进行了比对，并与其他DWV毒株进行了比较。
结果
这两种越南DWV毒株由10113个碱基对组成，包含一个2893个氨基酸的大的单一开放阅读框（ORF），起始于核苷酸1130，终止于核苷酸9812。对这两种DWV-VN毒株与其他DWV毒株进行了多核苷酸序列比对。DWV-VN毒株与几乎所有这些毒株的遗传同一性较低（从91.4%到92.0%），但与UK2的同一性更低（89.2%和89.4%），与China2毒株的同一性为（89.6%）。在中国3毒株（在……中）与DWV-VN毒株之间也观察到较低的同一性（91.7%和91.9%）。当将美国1、智利、意大利1、法国、英国1、英国2、日本、韩国2、中国1、中国2和中国3毒株与DWV-VN毒株进行比较时，推导的氨基酸序列比对显示出较高的遗传相似性（97.0%-97.9%）。当将韩国1毒株与DWV-SVN和DWV-NVN毒株进行比较时，该比例分别为96.7%和96.8%。在L、VP3和RdRp序列中鉴定出许多氨基酸替换。值得注意的是，我们在L蛋白中观察到六个替换，位于氨基酸27（E>I）、98（S>T）、120（A>V）、153（M>T）、170（D>F）和174（Y>F），VP3中有两个氨基酸变化，位于位置980（S>A）和1032（E>T），以及DWV-VN毒株特有的一个氨基酸变化，位于位置2627（R>C）。基于完整基因组序列、RdRp序列和Simplot分析的系统发育分析表明，……中的DWV-VN毒株与……中的DWV毒株之间存在显著差异。结果表明，……中DWV-VN毒株的遗传变异有助于它们适应地理条件，并可能导致DWV-VN毒株的病毒致病性发生变化。
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