Division of Plant Omics, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.
Department of Molecular Biology and Biotechnology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
J Basic Microbiol. 2021 Jan;61(1):37-44. doi: 10.1002/jobm.202000416. Epub 2020 Oct 1.
The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was found to utilize plastic as a carbon source. The Bacillus sonorensis SRCM101395 was used as a reference genome for mapping the reads. The genome size of strain AIIW2 was approximately 4.4 Mb and composed of 4737 coding sequences with 45.7% G + C contents. The whole genome comparison of strain AIIW2 with three closest Bacillus strains showed strain specificity, the 16S ribosomal RNA sequence shows 99.93% similarity with Bacillus paralicheniformis KJ-16 (KY694465). This genome data would provide the genetic basis in developing plastic bioremediation approaches and discover the enzymes pertinent in the biodegradation processes.
环境中塑料的地方性传播需要迫切需要采取可持续的方法。海洋微生物被发现具有巨大的生物活性，在海洋的生物地球化学循环中发挥着核心作用；然而，其中很少有微生物被探索用于生化循环或塑料降解。在本研究中，我们报告了海洋芽孢杆菌 AIIW2 的基因组草图序列，该菌被发现可以将塑料作为碳源利用。芽孢杆菌 sonorensis SRCM101395 被用作映射读数的参考基因组。菌株 AIIW2 的基因组大小约为 4.4 Mb，由 4737 个编码序列组成，GC 含量为 45.7%。菌株 AIIW2 与三个最接近的芽孢杆菌菌株的全基因组比较显示出菌株特异性，16S 核糖体 RNA 序列与 Bacillus paralicheniformis KJ-16 (KY694465) 具有 99.93%的相似性。该基因组数据将为开发塑料生物修复方法和发现与生物降解过程相关的酶提供遗传基础。
