Jeong Do-Won, Lee Byunghoon, Lee Hyundong, Jeong Keuncheol, Jang Mihyun, Lee Jong-Hoon
Department of Food and Nutrition, Dongduk Women's University, Seoul 02748, Republic of Korea.
Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
J Microbiol Biotechnol. 2018 Dec 28;28(12):1992-1998. doi: 10.4014/jmb.1809.09030.
In 2015, was separated from on the basis of phylogenomic and phylogenetic studies, and urease activity was reported as a phenotypic property able to differentiate between the two species. Subsequently, we have found that the urease activity of is strain-specific, and does not reliably discriminate between species, as strains having the same urease gene cluster were identified in and , the closest relatives of . We developed a multilocus sequence typing scheme using eight housekeeping genes, , and to clearly identify from closely related species and to find a molecular marker for the rapid identification of . The scheme differentiated 33 strains from 90 strains formerly identified as . Among the eight housekeeping genes, possesses appropriate polymorphic sites for the design of a -specific PCR primer set. The primer set designed in this study perfectly separated from and .
2015年，基于系统基因组学和系统发育研究，[具体物种A]与[具体物种B]被区分开来，并且脲酶活性被报告为一种能够区分这两个物种的表型特性。随后，我们发现[具体物种A]的脲酶活性具有菌株特异性，不能可靠地区分物种，因为在[具体物种A]的近亲[具体物种C]和[具体物种D]中鉴定出了具有相同脲酶基因簇的菌株。我们开发了一种多位点序列分型方案，使用八个管家基因[具体基因1]、[具体基因2]和[具体基因3]来明确区分[具体物种A]与密切相关的[具体物种B]类物种，并找到一种用于快速鉴定[具体物种A]的分子标记。该方案从先前鉴定为[具体物种B]的90个菌株中区分出33个[具体物种A]菌株。在这八个管家基因中，[具体基因4]具有适合设计[具体物种A]特异性PCR引物组的多态性位点。本研究设计的引物组完美地将[具体物种A]与[具体物种B]和[具体物种C]区分开来。
