Department of Microbiology, University of Hong Kong, Hong Kong, PR China.
Carol Yu Center for Infection, University of Hong Kong, Hong Kong, PR China.
J Gen Virol. 2013 Oct;94(Pt 10):2184-2190. doi: 10.1099/vir.0.052985-0. Epub 2013 Aug 5.
We describe the discovery and characterization of a novel paramyxovirus, porcine parainfluenza virus 1 (PPIV-1), from swine. The virus was detected in 12 (3.1 %) of 386 nasopharyngeal and two (0.7 %) of 303 rectal swab samples from 386 deceased pigs by reverse transcription-PCR, with viral loads of up to 10(6) copies ml(-1). Complete genome sequencing and phylogenetic analysis showed that PPIV-1 represented a novel paramyxovirus within the genus Respirovirus, being most closely related to human parainfluenza virus 1 (HPIV-1) and Sendai virus (SeV). In contrast to HPIV-1, PPIV-1 possessed a mRNA editing function in the phosphoprotein gene. Moreover, PPIV-1 was unique among respiroviruses in having two G residues instead of three to five G residues following the A6 run at the editing site. Nevertheless, PPIV-1, HPIV-1 and SeV share common genomic features and may belong to a separate group within the genus Respirovirus. The presence of PPIV-1 in mainly respiratory samples suggests a possible association with respiratory disease, similar to HPIV-1 and SeV.
我们描述了一种新型副黏病毒，猪副流感病毒 1（PPIV-1），来自猪。通过逆转录-PCR，在 386 份死亡猪的 386 份鼻咽拭子和 303 份直肠拭子样本中的 12 份（3.1%）和 2 份（0.7%）中检测到该病毒，病毒载量高达 10(6)拷贝/ml(-1)。完整基因组测序和系统发育分析表明，PPIV-1 是 Respirovirus 属中的一种新型副黏病毒，与人类副流感病毒 1（HPIV-1）和仙台病毒（SeV）最为密切相关。与 HPIV-1 不同，PPIV-1 在磷蛋白基因中具有 mRNA 编辑功能。此外，PPIV-1 在编辑位点后 A6 运行处有两个 G 而不是三个到五个 G 残基，这使其在呼肠孤病毒中是独一无二的。然而，PPIV-1、HPIV-1 和 SeV 具有共同的基因组特征，可能属于呼肠孤病毒属中的一个单独组。PPIV-1 主要存在于呼吸道样本中，这表明它可能与呼吸道疾病有关，类似于 HPIV-1 和 SeV。
