College of Marine Life Science, Ocean University of China, Qingdao, 266000, China.
Heze Institute for Food and Drug Control, Heze, 274000, China.
Adv Healthc Mater. 2020 Nov;9(21):e2000951. doi: 10.1002/adhm.202000951. Epub 2020 Oct 1.
Uncontrolled hemorrhage is the main reason of possible preventable death after accidental injury. It is necessary to develop a hemostatic agent with rapid hemostatic performance and good biocompatibility. In this study, a chitosan/diatom-biosilica-based aerogel is developed using dopamine as cross-linker by simple alkaline precipitation and tert-butyl alcohol replacement. The chitosan/diatom-biosilica aerogel exhibits favorable biocompatibility and multiscale hierarchical porous structure (from nanometer to micrometer), which can be controlled by the concentration of tert-butyl alcohol. The displacement of tert-butyl alcohol can keep the porosity of diatom-biosilica in aerogel and give it large surface with efficient water absorption ratio. 30% tert-butyl alcohol replacement of aerogel possesses the largest surface area (74.441 m g ), water absorption capacity (316.83 ± 2.04%), and excellent hemostatic performance in vitro blood coagulation (≈70 s). Furthermore, this aerogel exhibits the shortest clotting time and lowest blood loss in rat hemorrhage model. The strong interface effect between aerogel and blood is able to promote erythrocytes aggregation, platelets adhesion, and activation, as well as, activate the intrinsic coagulation pathway to accelerate blood coagulation. All the above results demonstrate that chitosan/diatom-biosilica aerogel has great potential to be a safe and rapid hemostatic material.
失控性出血是外伤性损伤后可能预防死亡的主要原因。有必要开发一种具有快速止血性能和良好生物相容性的止血剂。在本研究中，通过简单的碱性沉淀和叔丁醇取代法，以多巴胺为交联剂开发了一种壳聚糖/硅藻-生物硅基气凝胶。壳聚糖/硅藻-生物硅基气凝胶具有良好的生物相容性和多尺度分层多孔结构（从纳米到微米），可通过叔丁醇的浓度进行控制。叔丁醇的置换可以保持气凝胶中硅藻-生物硅的孔隙率，并赋予其大的表面积和高效的吸水率。30%叔丁醇置换的气凝胶具有最大的比表面积（74.441 m g）、吸水率（316.83±2.04%）和体外血液凝固的优异止血性能（≈70 s）。此外，该气凝胶在大鼠出血模型中表现出最短的凝血时间和最低的失血量。气凝胶与血液之间的强界面效应能够促进红细胞聚集、血小板黏附和激活，并激活内源性凝血途径，加速血液凝固。所有这些结果表明，壳聚糖/硅藻-生物硅基气凝胶具有成为安全、快速止血材料的巨大潜力。
