College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China.
Qingdao Stomatological Hospital, Department of Oral and Maxillofacial Surgery, No. 17 Dexian Road, Shinan District, Qingdao, Shandong, China.
Carbohydr Polym. 2020 May 15;236:116051. doi: 10.1016/j.carbpol.2020.116051. Epub 2020 Feb 21.
Effective bleeding control is critical first step in current civilian and military trauma treatment, however commercially available hemostatic materials are difficult to achieve expected effects. In this study, a composite sponge (H-D) based on hydroxybutyl chitosan (HBC) and diatom-biosilica (DB) was designed for hemorrhage control. H-D exhibited hierarchical porous structure, favorable biocompatibility (hemolysis ratio < 5 %, no cytotoxicity), along with high and fast fluid absorbability (11-16 times than that of weight), given effective hemostasis effect (clotting time shortened by 70 % than that of control). In vitro coagulation tests demonstrated that H-D could provide strong interface effect to induce erythrocyte absorption and aggregation, as well as activating the intrinsic coagulation pathway and thus accelerated blood coagulation. These results proved that H-D composite sponge has great potential for hemorrhage control.
有效的止血控制是当前民用和军事创伤治疗的关键第一步，然而，市售的止血材料很难达到预期的效果。在这项研究中，设计了一种基于羟丁基壳聚糖（HBC）和硅藻生物硅（DB）的复合海绵（H-D）用于控制出血。H-D 具有分级多孔结构，良好的生物相容性（溶血率<5%，无细胞毒性），以及高且快速的液体吸收能力（比自身重量高 11-16 倍），可实现有效的止血效果（凝血时间比对照组缩短了 70%）。体外凝血试验表明，H-D 可以提供强大的界面效应，诱导红细胞的吸收和聚集，并激活内在的凝血途径，从而加速血液凝固。这些结果证明 H-D 复合海绵在控制出血方面具有巨大的潜力。
