College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, Shandong Province, China.
Minimally invasive interventional therapy center, Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), 5# Donghai Middle Road, Qingdao 266000, Shandong Province, China.
J Colloid Interface Sci. 2023 Dec;651:544-557. doi: 10.1016/j.jcis.2023.07.202. Epub 2023 Jul 31.
Here, we developed a Ca modified diatom biosilica-based hemostat (DBp-Ca) with a full scale hierarchical porous structure (pore sizes range from micrometers to nanometers). The unique porous size in stepped arrangement of DBp-Cagive it selective adsorption capacity during coagulation process, resulted in rapid hemorrhage control. Based on in vitro and in vivo studies, it was confirmed that the primary micropores of DBp-Cagave it high porosity to hold water (water absorption: 78.46 ± 1.12 %) and protein (protein absorption: 83.7 ± 1.33 mg/g). Its secondary mesopores to macropores could reduce of water diffusion length to accelerate blood exchange (complete within 300 ms). The tertiary stacking pores of DBp-Ca could absorb platelets and erythrocytes to reduce more than 50 % of thrombosis time, and provided enough contact between Ca active site and coagulation factors for triggering clotting cascade reaction. This work not only developed a novel DBs based hemostat with efficient hemorrhage control, but also provided new insights to study procoagulant mechanism of inorganic hemostat with hierarchical porous structure from selective adsorption to rapid hemostasis.
在这里，我们开发了一种基于钙改性硅藻生物硅的止血剂（DBp-Ca），它具有全尺度的分级多孔结构（孔径范围从微米到纳米）。DBp-Ca 中阶梯式排列的独特多孔尺寸赋予了其在凝血过程中的选择性吸附能力，从而实现快速止血。通过体外和体内研究证实，DBp-Ca 的初级微孔赋予其高的吸水性（吸水率：78.46±1.12%）和蛋白质吸附能力（蛋白质吸附量：83.7±1.33mg/g）。其二级介孔和大孔可以减少水的扩散长度，从而加速血液交换（在 300ms 内完成）。DBp-Ca 的三级堆叠孔可以吸收血小板和红细胞，从而将血栓形成时间减少 50%以上，并为 Ca 活性位点和凝血因子之间提供足够的接触，以触发凝血级联反应。这项工作不仅开发了一种具有高效止血功能的新型基于 DBs 的止血剂，而且为研究具有分级多孔结构的无机止血剂的促凝机制提供了新的见解，从选择性吸附到快速止血。
