State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Mol Plant Microbe Interact. 2021 Jan;34(1):110-121. doi: 10.1094/MPMI-08-20-0227-R. Epub 2020 Nov 23.
is an ascomycetous fungus that causes grape anthracnose, a potentially devastating disease worldwide. In this study, a dual RNA-seq analysis was used to simultaneously monitor the fungal genes related to pathogenesis and grape genes related to defense during the interaction at 2, 3, 4, and 5 days postinoculation. Consistent with their potential roles in pathogenicity, genes for carbohydrate-active enzymes, secondary metabolite synthesis, pathogen-host interaction, and those encoding secreted proteins are upregulated during infection. Based on -mediated transient assays in , we further showed that eight and nine candidate effectors, respectively, suppressed BAX- and INF1-mediated programmed cell death. The host response was characterized by the induction of multiple defense systems against , including synthesis of phenylpropanoids, stilbenes, and terpenoid biosynthesis, cell-wall modifications, regulation by phytohormones, and expression of defense-related genes. Together, these findings offer new insights into molecular mechanisms underlying the grape- interaction.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
是一种子囊菌，可引起葡萄炭疽病，这是一种在世界范围内具有潜在破坏性的疾病。在这项研究中，采用双 RNA-seq 分析方法，在接种后 2、3、4 和 5 天，同时监测与发病机制相关的真菌基因和与防御相关的葡萄基因。与它们在致病性中的潜在作用一致，碳水化合物活性酶、次生代谢物合成、病原体-宿主相互作用的基因，以及编码分泌蛋白的基因在感染过程中上调。基于在 中进行的介导的瞬时测定，我们进一步表明，分别有 8 个和 9 个候选效应子抑制了 BAX 和 INF1 介导的程序性细胞死亡。宿主的反应特征是对 的多种防御系统的诱导，包括苯丙烷类、芪类和萜类生物合成、细胞壁修饰、植物激素调控以及防御相关基因的表达。总之，这些发现为葡萄与互作的分子机制提供了新的见解。
