College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No. 2888, Changchun 130118, PR China.
Jilin Medical University, Jilin Street No. 5, Jilin 132013, PR China.
Pol J Vet Sci. 2020 Sep;23(3):405-414. doi: 10.24425/pjvs.2020.134685.
MDAP-2 is a new AMP with high inhibitory activity on Salmonella gallinarum, which may be developed as an antimicrobial agent in the agricultural industry and food preservation. To investigate the underlying the action mechanism of MDAP-2 on Salmonella gallinarum, impacts of MDAP-2 on the growth curve and bacterial morphology of Salmonella gallinarum were studied. iTRAQ-based proteomics analysis was also performed on proteins extracted from treated and untreated Salmonella gallinarum cells. The differentially expressed proteins were then analyzed using the KEGG and GO databases. Finally, the function of some differentially expressed proteins was verified. The results showed that 150 proteins (41 up-regulated and 109 down-regulated) were found differentially expressed (fold > 1.8, p⟨0.05). The results indi- cate that MDAP-2 kills Salmonella gallinarum mainly through two mechanisms: (i) direct inhibi- tion of cell wall/ membrane/ envelope biogenesis, energy production/ conversion, carbohydrate transport/ metabolism, and DNA transcription/ translation through regulation of special protein levels; (ii) indirect effects on the same pathway through the accumulation of Reactive oxygen species (O2 ▪-, H2O2 and OH▪-).
MDAP-2 是一种对鸡白痢沙门氏菌具有高抑制活性的新型 AMP，可能被开发为农业和食品保鲜领域的抗菌剂。为了研究 MDAP-2 对鸡白痢沙门氏菌的作用机制，本研究考察了 MDAP-2 对鸡白痢沙门氏菌生长曲线和细菌形态的影响。还对经 MDAP-2 处理和未经处理的鸡白痢沙门氏菌细胞提取的蛋白质进行了 iTRAQ 基于蛋白质组学分析。然后使用 KEGG 和 GO 数据库对差异表达蛋白进行分析。最后，验证了一些差异表达蛋白的功能。结果表明，发现 150 种蛋白质（41 种上调和 109 种下调）表达差异（倍数>1.8，p<0.05）。结果表明，MDAP-2 通过两种机制杀死鸡白痢沙门氏菌：（i）通过调节特殊蛋白质水平，直接抑制细胞壁/膜/包膜生物发生、能量产生/转化、碳水化合物运输/代谢以及 DNA 转录/翻译；（ii）通过活性氧（O2▪-、H2O2 和 OH▪-）的积累对同一途径产生间接影响。
