Department of Reproductive Center, the 940th Hospital of Joint Logistics Support Force of Chinese PLA, Lanzhou, 730050, P. R. China.
Department of Health Service, the 940th Hospital of Joint Logistics Support Force of Chinese PLA, Lanzhou, 730050, P. R. China.
Chem Biodivers. 2020 Nov;17(11):e2000671. doi: 10.1002/cbdv.202000671. Epub 2020 Nov 3.
Cisplatin (CISP) is an efficacious anticancer agent used in chemotherapy, however, the constraint to its clinical utility is the stray organ toxicity including testicular damage linked to oxidative and inflammatory cascades. This study aimed to explore the protective effect of nucleosides-rich extract from Cordyceps cicadae (NRCE) against CISP-induced testicular damage in rats. Rats were subjected to prophylactic oral administration of NRCE (50, 100 and 400 mg/kg body weight/day) for 7 days prior to testicular toxicity induced by CISP (10 mg/kg, ip) and were sacrificed after 72 h post-CISP injection. Cisplatin caused significant deficits in sperm count, viability and motility, testosterone and follicle stimulating hormone (FSH) compared to normal control. It depressed testicular activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), total antioxidant content (TAC), whereas malondialdehyde (MDA) increased remarkably. CISP considerably increased tumor necrosis factor-alpha (TNF-α) and interleukin-one beta (IL-1β) with alterations in testis histology compared to normal control. Interestingly, NRCE pretreatment inhibited the CISP-induced alterations in reproductive indices, restored the antioxidant activities in testes as well as inflammatory mediators and histology comparable to control. Our findings demonstrate that NRCE could prevent CISP testicular damage via inhibition of oxidative stress and pro-inflammation in rats.
顺铂（CISP）是一种有效的抗癌药物，用于化疗，然而，其临床应用的限制是偶然的器官毒性，包括与氧化和炎症级联相关的睾丸损伤。本研究旨在探讨虫草核苷丰富提取物（NRCE）对大鼠顺铂诱导睾丸损伤的保护作用。大鼠在顺铂（10mg/kg，ip）诱导睾丸毒性前 7 天进行预防性口服 NRCE（50、100 和 400mg/kg 体重/天）给药，并在注射顺铂后 72 小时处死。与正常对照组相比，顺铂导致精子计数、活力和运动能力、睾酮和卵泡刺激素（FSH）显著降低。它降低了睾丸谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、总抗氧化能力（TAC）的活性，而丙二醛（MDA）显著增加。与正常对照组相比，顺铂显著增加了肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），并改变了睾丸组织学。有趣的是，NRCE 预处理抑制了 CISP 诱导的生殖指数改变，恢复了睾丸的抗氧化活性以及炎症介质和组织学，与对照组相当。我们的研究结果表明，NRCE 可以通过抑制氧化应激和炎症在大鼠中预防 CISP 睾丸损伤。
