Department of Laboratory Medicine, Lund University, 22184 Lund, Sweden.
Viruses. 2020 Sep 30;12(10):1110. doi: 10.3390/v12101110.
Human papillomaviruses (HPVs) depend on the cellular RNA-processing machineries including alternative RNA splicing and polyadenylation to coordinate HPV gene expression. HPV RNA processing is controlled by cis-regulatory RNA elements and trans-regulatory factors since the HPV splice sites are suboptimal. The definition of HPV exons and introns may differ between individual HPV mRNA species and is complicated by the fact that many HPV protein-coding sequences overlap. The formation of HPV ribonucleoproteins consisting of HPV pre-mRNAs and multiple cellular RNA-binding proteins may result in the different outcomes of HPV gene expression, which contributes to the HPV life cycle progression and HPV-associated cancer development. In this review, we summarize the regulation of HPV16 gene expression at the level of RNA processing with focus on the interactions between HPV16 pre-mRNAs and cellular RNA-binding factors.
人乳头瘤病毒（HPV）依赖于细胞 RNA 处理机制，包括选择性 RNA 剪接和多聚腺苷酸化，以协调 HPV 基因表达。由于 HPV 剪接位点不佳，HPV RNA 处理受到顺式调节 RNA 元件和反式调节因子的控制。HPV 外显子和内含子的定义在个体 HPV mRNA 物种之间可能不同，并且由于许多 HPV 蛋白编码序列重叠，这一情况变得更加复杂。由 HPV 前体 RNA 和多种细胞 RNA 结合蛋白组成的 HPV 核糖核蛋白的形成可能导致 HPV 基因表达的不同结果，这有助于 HPV 生命周期的进展和 HPV 相关癌症的发展。在这篇综述中，我们总结了 HPV16 基因表达在 RNA 处理水平上的调控，重点介绍了 HPV16 前体 RNA 与细胞 RNA 结合因子之间的相互作用。
