氧气和一氧化氮对碱性磷酸酶磷光的猝灭。蛋白质结构刚性区域的证据。
Quenching of alkaline phosphatase phosphorescence by O2 and NO. Evidence for inflexible regions of protein structure.
作者信息
Strambini G B
出版信息
Biophys J. 1987 Jul;52(1):23-8. doi: 10.1016/S0006-3495(87)83184-3.
Abstract
The rate constant for quenching the phosphorescence of alkaline phosphatase by molecular oxygen was measured as a function of temperature. The results disagree with previous determinations and, contrary to fluorescence quenching, show that diffusion of O2 to this region of the macromolecule is a highly hindered process. When nitric oxide is introduced as a quencher, similarly small rate constants were found. While the activation energy for this process is identical for both quenchers, it is much smaller than for structural fluctuations at the chromophore site as manifested by the intrinsic triplet-state lifetime. These findings are analyzed in terms of a mechanism that takes into account static quenching at large distances and does not require penetration of the quencher all the way to the chromophore.
摘要
测定了分子氧猝灭碱性磷酸酶磷光的速率常数与温度的函数关系。结果与先前的测定结果不一致，并且与荧光猝灭相反，表明氧气扩散到该大分子区域是一个高度受阻的过程。当引入一氧化氮作为猝灭剂时，也发现了类似的小速率常数。虽然这两种猝灭剂在此过程中的活化能相同，但比发色团位点的结构波动所表现出的本征三重态寿命的活化能小得多。根据一种考虑远距离静态猝灭且不需要猝灭剂一直穿透到发色团的机制对这些发现进行了分析。
相似文献
1
Quenching of alkaline phosphatase phosphorescence by O2 and NO. Evidence for inflexible regions of protein structure.氧气和一氧化氮对碱性磷酸酶磷光的猝灭。蛋白质结构刚性区域的证据。
Biophys J. 1987 Jul;52(1):23-8. doi: 10.1016/S0006-3495(87)83184-3.
2
Photophysics of indole-2-carboxylic acid (I2C) and indole-5-carboxylic acid (I5C): heavy atom effect.吲哚-2-羧酸（I2C）和吲哚-5-羧酸（I5C）的光物理性质：重原子效应。
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Dec;116:183-95. doi: 10.1016/j.saa.2013.07.011. Epub 2013 Jul 18.
3
Penetration of dioxygen into proteins studied by quenching of phosphorescence and fluorescence.通过磷光和荧光猝灭研究氧气在蛋白质中的渗透。
Biochemistry. 1983 Mar 29;22(7):1526-32. doi: 10.1021/bi00276a002.
4
Quenching of room temperature protein phosphorescence by added small molecules.添加小分子对室温蛋白质磷光的猝灭作用。
Biochemistry. 1988 Nov 1;27(22):8466-74. doi: 10.1021/bi00422a026.
5
Effect of heavy water on protein flexibility.重水对蛋白质柔韧性的影响。
Biophys J. 2002 Jun;82(6):3246-53. doi: 10.1016/S0006-3495(02)75666-X.
6
Amplitude spectrum of structural fluctuations in proteins from the internal diffusion of solutes of increasing molecular size: a Trp phosphorescence quenching study.蛋白质结构波动的振幅谱：从溶质内部扩散到增加的分子大小：色氨酸磷光猝灭研究。
Biochemistry. 2011 Feb 15;50(6):970-80. doi: 10.1021/bi101738w. Epub 2011 Jan 25.
7
Quenching of tryptophan phosphorescence in Escherichia coli alkaline phosphatase by long-range transfer mechanisms to external agents in the rapid-diffusion limit.在快速扩散极限下，通过长程转移机制将大肠杆菌碱性磷酸酶中的色氨酸磷光猝灭至外部试剂。
Biochemistry. 1991 Jan 22;30(3):668-75. doi: 10.1021/bi00217a012.
8
Study on Escherichia coli alkaline phosphatase conformation by phosphorimetry in the presence of denaturant.
Spectrochim Acta A Mol Biomol Spectrosc. 2003 Nov;59(13):3185-91. doi: 10.1016/s1386-1425(03)00137-9.
9
Gated quenching of intrinsic fluorescence and phosphorescence of globular proteins. An extended model.球状蛋白质固有荧光和磷光的门控猝灭。一个扩展模型。
Biophys J. 1986 Jul;50(1):55-61. doi: 10.1016/S0006-3495(86)83438-5.
10
Quenching of the zinc-protoporphyrin triplet state as a measure of small-molecule diffusion through the structure of myoglobin.
Biochemistry. 1987 Jun 2;26(11):3240-4. doi: 10.1021/bi00385a046.
引用本文的文献
1
Tryptophan phosphorescence of ribonuclease T1 as a probe of protein flexibility.核糖核酸酶 T1 色氨酸磷光作为蛋白质柔性的探针。
J Fluoresc. 1992 Sep;2(3):157-65. doi: 10.1007/BF00866930.
2
Acrylonitrile quenching of trp phosphorescence in proteins: a probe of the internal flexibility of the globular fold.丙烯腈猝灭蛋白质中色氨酸磷光：研究球状折叠内部柔韧性的探针。
Biophys J. 2010 Aug 4;99(3):944-52. doi: 10.1016/j.bpj.2010.05.022.
3
Multiple unfolding intermediates of human placental alkaline phosphatase in equilibrium urea denaturation.人胎盘碱性磷酸酶在平衡尿素变性中的多个解折叠中间体
Biophys J. 2001 Dec;81(6):3456-71. doi: 10.1016/S0006-3495(01)75977-2.
4
Structural perturbations of azurin deposited on solid matrices as revealed by trp phosphorescence.通过色氨酸磷光揭示的沉积在固体基质上的天青蛋白的结构扰动。
Biophys J. 2001 May;80(5):2431-8. doi: 10.1016/S0006-3495(01)76212-1.
5
Glycerol effects on protein flexibility: a tryptophan phosphorescence study.甘油对蛋白质柔韧性的影响：一项色氨酸磷光研究。
Biophys J. 1993 Jul;65(1):131-7. doi: 10.1016/S0006-3495(93)81069-5.
6
Evidence for ligand-induced conformational changes in proteins from phosphorescence spectroscopy.来自磷光光谱法的蛋白质中配体诱导构象变化的证据。
Biophys J. 1989 Aug;56(2):353-60. doi: 10.1016/S0006-3495(89)82681-5.
7
Long-range electron exchange measured in proteins by quenching of tryptophan phosphorescence.通过色氨酸磷光猝灭测量蛋白质中的长程电子交换。
Proc Natl Acad Sci U S A. 1990 Jul;87(13):5099-103. doi: 10.1073/pnas.87.13.5099.
8
Time-resolved circularly polarized protein phosphorescence.时间分辨圆偏振蛋白质磷光
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10154-8. doi: 10.1073/pnas.89.21.10154.
本文引用的文献
1
Kinetic studies of liver alcohol dehydrogenase.肝脏乙醇脱氢酶的动力学研究
Biochem J. 1962 Aug;84(2):244-54. doi: 10.1042/bj0840244.
2
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.大肠杆菌碱性磷酸酶的精细结构遗传学与化学研究。I. 碱性磷酸酶的纯化与特性分析
Biochim Biophys Acta. 1960 Mar 11;38:470-83. doi: 10.1016/0006-3002(60)91282-8.
3
A model of dynamic quenching of fluorescence in globular proteins.球状蛋白中荧光动态猝灭的模型
Biophys J. 1984 Apr;45(4):789-94. doi: 10.1016/S0006-3495(84)84223-X.
4
Fluorescence quenching of Trp-314 of liver alcohol dehydrogenase by oxygen.氧对肝脏乙醇脱氢酶色氨酸-314的荧光猝灭作用。
Biophys Chem. 1984 Oct;20(3):201-7. doi: 10.1016/0301-4622(84)87024-6.
5
Intrinsic phosphorescence from proteins in the solid state.
Photochem Photobiol. 1984 Jun;39(6):725-9.
6
The three dimensional structure of alkaline phosphatase from E. coli.来自大肠杆菌的碱性磷酸酶的三维结构。
Adv Enzymol Relat Areas Mol Biol. 1983;55:453-80. doi: 10.1002/9780470123010.ch6.
7
Singular oxygen effects on the room-temperature phosphorescence of alcohol dehydrogenase from horse liver.单线态氧对马肝醇脱氢酶室温磷光的影响。
Biophys J. 1983 Jul;43(1):127-30. doi: 10.1016/S0006-3495(83)84331-8.
8
Penetration of dioxygen into proteins studied by quenching of phosphorescence and fluorescence.通过磷光和荧光猝灭研究氧气在蛋白质中的渗透。
Biochemistry. 1983 Mar 29;22(7):1526-32. doi: 10.1021/bi00276a002.
9
Diffusion of oxygen in water and hydrocarbons using an electron spin resonance spin-label technique.利用电子自旋共振自旋标记技术研究氧气在水和碳氢化合物中的扩散
Biophys J. 1984 Apr;45(4):743-8. doi: 10.1016/S0006-3495(84)84217-4.
10
Oxygen quenching of pyrenebutyric acid fluorescence in water. A dynamic probe of the microenvironment.水中芘丁酸荧光的氧猝灭：微环境的动态探针
Biochemistry. 1970 Feb 3;9(3):464-73. doi: 10.1021/bi00805a003.
Zotero 插件