Krairojananan Panadda, Thaipadungpanit Janjira, Leepitakrat Surachai, Monkanna Taweesak, Wanja Elizabeth W, Schuster Anthony L, Costa Federico, Poole-Smith B Katherine, McCardle Patrick W
Department of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, Thailand.
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Trop Med Infect Dis. 2020 Sep 30;5(4):154. doi: 10.3390/tropicalmed5040154.
Leptospirosis is a neglected zoonotic disease affecting mostly the world's tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and carriage rate of pathogenic in rodent reservoirs to leptospirosis incidence, we surveyed rodents, between 2011 and 2012, in four provinces in northeastern Thailand with the highest incidence rates of human leptospirosis cases. We used real-time PCR to detect pathogenic in rodent kidneys, partial 16S rRNA gene sequencing to classify the infecting species, and whole 16S rDNA sequencing to classify species of isolated . Overall prevalence of infection was 3.6% (18/495). Among infected rodents, (14.3%), (3.6%), and . (3.2%) had renal carriage. We identified two pathogenic species: . ( = 15) and . ( = 3). In addition, an . (LS0914U) isolate was recovered from the urine of . . infection was more prevalent in low density rodent populations, such as . . In contrast, there was a lower prevalence of infection in high density rodent populations of . and . .
钩端螺旋体病是一种被忽视的人畜共患病，主要影响世界热带地区。泰国东北部的农村人口遭受大量钩端螺旋体感染，他们丰富的稻田是理想的啮齿动物栖息地。为了评估啮齿动物宿主中致病性钩端螺旋体的多样性和携带率对钩端螺旋体病发病率的影响，我们在2011年至2012年期间对泰国东北部人类钩端螺旋体病发病率最高的四个省份的啮齿动物进行了调查。我们使用实时PCR检测啮齿动物肾脏中的致病性钩端螺旋体，通过部分16S rRNA基因测序对感染的钩端螺旋体物种进行分类，并通过全16S rDNA测序对分离出的钩端螺旋体物种进行分类。钩端螺旋体感染的总体患病率为3.6%（18/495）。在受感染的啮齿动物中，（14.3%）、（3.6%）和。（3.2%）有肾脏携带。我们鉴定出两种致病性钩端螺旋体物种：。（=15）和。（=3）。此外，从。的尿液中分离出一株。（LS0914U）。钩端螺旋体感染在低密度啮齿动物种群中更为普遍，如。。相比之下，在。和。的高密度啮齿动物种群中，钩端螺旋体感染的患病率较低。
