的低表达促进骨肉瘤患者的细胞增殖并具有预后价值。 （注：原文中“Low-expression of ”后面缺少具体内容）
Low-expression of promotes cell proliferation and exhibits prognostic value in osteosarcoma patients.
作者信息
Liu Shouying, Zhou Changxi, Zhu Changbao, Song Qiuhe, Wen Ming, Liu Ye, An Huaijie
机构信息
Department of Orthopaedics, The 253th Hospital of The Chinese People's Liberation Army Hohhot 010051, China.
Department of Nanlou Respiratory Diseases, PLA General Hospital Beijing 100853, China.
出版信息
Int J Clin Exp Pathol. 2017 Aug 1;10(8):9035-9041. eCollection 2017.
AIM
) play important roles in occurrence and development of osteosarcoma. Previous studies had verified the role of ) in various diseases, especially in cancers. Our purpose in this study was to investigate the values of in development and prognosis of osteosarcoma.
METHODS
QRT-PCR was used to measure the expression of in osteosarcoma tissues, adjacent tissues and healthy tissues as well as in osteosarcoma cell lines MG63, U2OS and normal osteoblastic cell line hFOB1.19. CCK-8 and siRNA assays were performed to estimate the effect of in the process of cell proliferation. The Kaplan-Meier and Cox regression analysis were performed to detect the prognostic values of the in osteosarcoma patients.
RESULTS
The results demonstrated that expression decreased in osteosarcoma tissues and cell lines compared with the controls. Proliferation assay declared that the cell proliferation was accelerated by down-regulation of . Kaplan-Meier exhibited that the overall survival time of low- expression was shorter than those with high- expression (=0.001). Cox regression analysis revealed that Enneking, distant metastasis and recurrence were all prognostic factors just like low-.
CONCLUSION
The expression of was lower in osteosarcoma tissues and cell lines and acted as a tumor suppressor. The low-expression of was associated with clinicopathologic characteristics (age, tumor site, Enneking, therapies). Moreover, might be an independent prognostic marker and promote cell proliferation in osteosarcoma.
目的
（此处原文有缺失内容）在骨肉瘤的发生和发展中起重要作用。先前的研究已经证实了（此处原文有缺失内容）在各种疾病，尤其是癌症中的作用。本研究的目的是探讨（此处原文有缺失内容）在骨肉瘤发展和预后中的价值。
方法
采用定量逆转录聚合酶链反应（QRT-PCR）检测骨肉瘤组织、癌旁组织和健康组织以及骨肉瘤细胞系MG63、U2OS和正常成骨细胞系hFOB1.19中（此处原文有缺失内容）的表达。进行细胞计数试剂盒-8（CCK-8）和小干扰RNA（siRNA）检测以评估（此处原文有缺失内容）在细胞增殖过程中的作用。进行Kaplan-Meier和Cox回归分析以检测（此处原文有缺失内容）在骨肉瘤患者中的预后价值。
结果
结果表明，与对照组相比，骨肉瘤组织和细胞系中（此处原文有缺失内容）表达降低。增殖检测表明，（此处原文有缺失内容）下调可加速细胞增殖。Kaplan-Meier分析显示，低（此处原文有缺失内容）表达者的总生存时间短于高（此处原文有缺失内容）表达者（P = 0.001）。Cox回归分析显示，Enneking分期、远处转移和复发与低（此处原文有缺失内容）表达一样，均为预后因素。
结论
骨肉瘤组织和细胞系中（此处原文有缺失内容）表达较低，（此处原文有缺失内容）起肿瘤抑制作用。（此处原文有缺失内容）低表达与临床病理特征（年龄、肿瘤部位、Enneking分期、治疗方法）相关。此外，（此处原文有缺失内容）可能是骨肉瘤的独立预后标志物，并促进骨肉瘤细胞增殖。
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