Health and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, 5000, Australia.
Quality Use of Medicines and Pharmacy Research Centre, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, 5000, Australia.
Plant Foods Hum Nutr. 2020 Dec;75(4):621-627. doi: 10.1007/s11130-020-00858-4. Epub 2020 Oct 3.
Advanced glycation end-products (AGEs) may be a contributing factor in the development of diabetes-specific vascular pathologies that affect the retina, glomerulus and peripheral nerves. In this study, Australian native food plant species Syzygium paniculatum was investigated for activities relevant to Type 2 diabetes mellitus including inhibition of α-amylase, α-glucosidase and protein glycation. A methanolic extract of the leaves showed the strongest α-amylase inhibition (IC = 14.29 ± 0.82 μg/mL, p < 0.05) when compared with other extracts. For inhibition of α-glucosidase, the strongest inhibition was shown for the water, methanolic and acetone extracts of leaves with IC values ranging from 4.73 ± 0.96 to 7.26 ± 0.92 μg/mL. In the BSA-glucose model, fruit and leaf extracts inhibited formation of protein-bound fluorescent AGEs with IC values ranging between 11.82 ± 0.71 and 96.80 ± 13.41 μg/mL. Pearson's correlation analysis showed that the AGE inhibition significantly correlated with DPPH (r = -0.8964, p < 0.05) and ABTS (r = -0.8326, p < 0.05). α-amylase inhibitory activities strongly correlated with DPPH (r = -0.8964, p < 0.001). α-glucosidase inhibition strongly correlated with TPC (r = -0.9243, p < 0.05), FRAP (r = -0.9502, p < 0.01), DPPH (r = -0.9317, p < 0.01) and ABTS (r = -0.9486, p < 0.01). This study provides a strong rationale for further investigation aimed at isolating and identifying the active compounds responsible for the observed effects on targets relevant to diabetes.
晚期糖基化终产物（AGEs）可能是导致糖尿病特异性血管病变的一个因素，这些病变会影响视网膜、肾小球和周围神经。在这项研究中，澳大利亚本土植物物种 Syzygium paniculatum 的叶片提取物被用于研究与 2 型糖尿病相关的活性，包括抑制α-淀粉酶、α-葡萄糖苷酶和蛋白质糖化。与其他提取物相比，该叶片提取物对α-淀粉酶的抑制作用最强（IC = 14.29 ± 0.82 μg/mL，p < 0.05）。对于α-葡萄糖苷酶的抑制作用，水提物、甲醇提物和丙酮提物对叶片的抑制作用最强，IC 值范围为 4.73 ± 0.96 至 7.26 ± 0.92 μg/mL。在 BSA-葡萄糖模型中，果实和叶片提取物抑制形成蛋白结合的荧光 AGEs，IC 值范围为 11.82 ± 0.71 至 96.80 ± 13.41 μg/mL。Pearson 相关分析显示，AGE 抑制作用与 DPPH（r = -0.8964，p < 0.05）和 ABTS（r = -0.8326，p < 0.05）显著相关。α-淀粉酶抑制活性与 DPPH 显著相关（r = -0.8964，p < 0.001）。α-葡萄糖苷酶抑制作用与 TPC（r = -0.9243，p < 0.05）、FRAP（r = -0.9502，p < 0.01）、DPPH（r = -0.9317，p < 0.01）和 ABTS（r = -0.9486，p < 0.01）显著相关。这项研究为进一步分离和鉴定对糖尿病相关靶点具有观察到的作用的活性化合物提供了强有力的依据。
