鉴定 NCAPH 作为乳腺癌预后的生物标志物。

Identification of NCAPH as a biomarker for prognosis of breast cancer.

机构信息

College of Medicine, Qingdao University, Qingdao, 266071, China.

Department of Human Anatomy, Histology and Embryology, College of Medicine, Qingdao University, Qingdao, 266071, China.

出版信息

Mol Biol Rep. 2020 Oct;47(10):7831-7842. doi: 10.1007/s11033-020-05859-9. Epub 2020 Oct 3.

DOI:10.1007/s11033-020-05859-9

PMID:33009967

Abstract

Non-SMC condensin I complex subunit H (NCAPH) is a structural component of chromosomes during mitosis, which up-regulates in various cancers. However, the role of NCAPH in breast cancer still hasn't been clearly explored. Our aim is to confirm the value of NCAPH as a prognostic biomarker of breast cancer. We revealed the high expression of NCAPH in breast cancer by using Oncomine, Ualcan and GOBO analysis. Quantitative Real-time PCR (qRT-PCR) analysis was conducted to detect the mRNA expression of NCAPH in MCF-7 and MCF-10A cells, and thus higher mRNA level of NCAPH in MCF-7 cells was confirmed. STRING and gene enrich analysis were conducted to explore the interaction between NCAPH and other proteins, hence the functionality of NCAPH in mitosis is demonstrated. With Kaplan-Meier plotter we found that upregulation of NCAPH is indicative of a poor prognosis especially in hormone receptor positive breast cancer. We confirmed the potential competence of NCAPH as a promising biomarker in breast cancer prognosis.

摘要

非 SMC 凝聚素 I 复合物亚基 H（NCAPH）是有丝分裂过程中染色体的结构组成部分，在各种癌症中上调。然而，NCAPH 在乳腺癌中的作用仍未得到明确探索。我们的目的是确认 NCAPH 作为乳腺癌预后生物标志物的价值。我们使用 Oncomine、Ualcan 和 GOBO 分析揭示了 NCAPH 在乳腺癌中的高表达。通过定量实时 PCR（qRT-PCR）分析检测 MCF-7 和 MCF-10A 细胞中 NCAPH 的 mRNA 表达，证实 MCF-7 细胞中 NCAPH 的 mRNA 水平更高。通过 STRING 和基因富集分析探索 NCAPH 与其他蛋白质之间的相互作用，从而证明了 NCAPH 在有丝分裂中的功能。通过 Kaplan-Meier plotter 分析发现，NCAPH 的上调预示着预后不良，尤其是在激素受体阳性乳腺癌中。我们证实了 NCAPH 作为乳腺癌预后有前途的生物标志物的潜在能力。