NCAP 家族基因与人类肉瘤预后和免疫浸润的关联。

Association of NCAP family genes with prognosis and immune infiltration of human sarcoma.

机构信息

Department of Orthopedics, People’s Hospital of Pingchang County, Pingchang, Sichuan 636400, China.

Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.

出版信息

Aging (Albany NY). 2023 May 9;15(10):4108-4121. doi: 10.18632/aging.204683.

DOI:10.18632/aging.204683

PMID:37192046

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10258000/

Abstract

OBJECTIVE

This study was conducted to explore the correlation of NCAP family genes with expression, prognosis, and immune infiltration in human sarcoma.

RESULTS

Compared with normal human tissues, six NCAP family genes were highly expressed in sarcoma tissues, and high expression of the six genes were significantly associated with the poor prognosis of sarcoma patients. The expression of NCAPs in sarcoma was significantly related to the low infiltration level of macrophages and CD4+ T cells. GO and KEGG enrichment analysis showed that NCAPs and their interacting genes were mainly enriched in organelle fission for biological processes (BP), spindle for cellular component (CC), tubulin binding for molecular function (MF), and 'Cell cycle' pathway.

METHODS

We explored the expression of NCAP family members by ONCOMINE, and GEPIA databases. Additionally, the prognostic value of NCAP family genes in sarcoma was detected by Kaplan-Meier Plotter and GEPIA databases. Moreover, we explored the relationship between NCAP family gene expression level and immune infiltration using the TIMER database. Finally, we performed GO and KEGG analysis for NCAPs-related genes by DAVID database.

CONCLUSION

The six members of NCAP gene family can be used as biomarkers to predict the prognosis of sarcoma. They were also correlated with the low immune infiltration in sarcoma.

摘要

目的

本研究旨在探讨 NCAP 家族基因与人类肉瘤表达、预后和免疫浸润的相关性。

结果

与正常人体组织相比，NCAP 家族的 6 个基因在肉瘤组织中高表达，且这 6 个基因的高表达与肉瘤患者的不良预后显著相关。肉瘤中 NCAPs 的表达与巨噬细胞和 CD4+T 细胞浸润水平低显著相关。GO 和 KEGG 富集分析显示，NCAPs 及其相互作用基因主要富集于生物学过程（BP）中的细胞器分裂、细胞成分（CC）中的纺锤体、分子功能（MF）中的微管结合以及“细胞周期”通路。

方法

我们通过 ONCOMINE 和 GEPIA 数据库探索 NCAP 家族成员的表达情况。此外，我们通过 Kaplan-Meier Plotter 和 GEPIA 数据库检测 NCAP 家族基因在肉瘤中的预后价值。并且，我们使用 TIMER 数据库探索 NCAP 家族基因表达水平与免疫浸润之间的关系。最后，我们通过 DAVID 数据库对 NCAP 相关基因进行 GO 和 KEGG 分析。