School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Diseases, China Medical University, Shenyang, China.
FEBS Open Bio. 2020 Nov;10(11):2404-2416. doi: 10.1002/2211-5463.12991. Epub 2020 Oct 21.
Fluorosis is a common disease characterized by disruptions in bone metabolism and enamel development. The production of reactive oxygen species is thought to play an important role in fluorosis. Gastrodin (4-hydroxybenzylalcohol4-O-beta-D-glucopyranoside) has been reported to have antioxidative activity, and so here we examined whether gastrodin has protective effects against oxidative stress and bone tissue toxicity in rats with fluorosis. Wistar rats were given different doses of gastrodin 1 month after fluoride administration, and samples of blood, bone and teeth were collected after 2, 3 and 4 months; glutathione peroxidase glu, CAT and SOD levels in the fluorosis group were lower than those in the control group. Gastrodin treatment in rats ameliorated oxidative stress and fluoride accumulation that were induced by fluoride; treatment with 400 mg·kg gastrodin protected trabecular bone structure and reduced femur and alveolar bone injury in rats with fluorosis. Enhanced expression of cysteinyl aspartate-specific proteinase (caspase) 3, caspase-9 and Bax and decreased expression of Bcl-2 induced by fluoride were also reversed by gastrodin. In summary, the present data suggest that gastrodin, and in particular a dose of 400 mg·kg , can improve the antioxidative capacity of rats, reduce concentration of fluoride in tissues, alleviate bone damage and modulate expression of Bcl-2, Bax, caspase-3 and caspase-9.
氟中毒是一种常见疾病，其特征是骨代谢和牙釉质发育紊乱。活性氧的产生被认为在氟中毒中起重要作用。天麻素（4-羟基苯甲醇 4-O-β-D-吡喃葡萄糖苷）已被报道具有抗氧化活性，因此我们在这里研究了天麻素是否对氟中毒大鼠的氧化应激和骨组织毒性具有保护作用。在给予氟化物 1 个月后，Wistar 大鼠给予不同剂量的天麻素，在 2、3 和 4 个月后收集血液、骨骼和牙齿样本；氟中毒组的谷胱甘肽过氧化物酶 glu、CAT 和 SOD 水平低于对照组。天麻素治疗可改善氟化物诱导的氧化应激和氟化物蓄积；400mg·kg 的天麻素治疗可保护氟中毒大鼠的小梁骨结构，减少股骨和牙槽骨损伤。天麻素还逆转了氟化物诱导的半胱天冬氨酸特异性蛋白酶（caspase）3、caspase-9 和 Bax 的表达增强以及 Bcl-2 的表达减少。综上所述，本研究数据表明，天麻素，特别是 400mg·kg 的剂量，可以提高大鼠的抗氧化能力，降低组织中氟化物的浓度，减轻骨损伤，并调节 Bcl-2、Bax、caspase-3 和 caspase-9 的表达。
