Li K Q, Jia S S, Ma M, Shen H Z, Xu L, Liu G P, Huang S Y, Zhang D S
Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Braz J Med Biol Res. 2016 Jul 11;49(8). doi: 10.1590/1414-431X20165291.
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP activity and calcium content were significantly enhanced by 10 μmol/L NaF with osteogenic inductive medium. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. We conclude that moderate concentrations of NaF can stimulate proliferation and mineralization in periodontal ligament cells. These in vitro findings may provide a novel therapeutic approach for acceleration of periodontal regeneration by addition of suitable concentrations of NaF into the medication for periodontitis treatment, i.e., into periodontal packs and tissue patches.
氟化物通常添加在牙膏或漱口水中以保护牙齿免受龋齿侵害，它可能是一种促进牙周再生的新型治疗方法。因此，我们在体外研究了氟化物对人牙周膜细胞增殖和矿化的影响。将不同浓度的氟化钠添加到成骨诱导培养基中刺激牙周膜细胞。进行了细胞鉴定的免疫组织化学、细胞增殖、碱性磷酸酶（ALP）活性测定、茜素红S染色和定量实时聚合酶链反应（RT-PCR）。中等浓度的氟化钠（5（0）-500 μmol/L）具有促增殖作用，其中500 μmol/L效果最佳。10 μmol/L氟化钠与成骨诱导培养基一起可显著提高ALP活性和钙含量。由于氟化钠浓度和处理时间不同，定量RT-PCR数据在基因方面存在差异。我们得出结论，中等浓度的氟化钠可刺激牙周膜细胞的增殖和矿化。这些体外研究结果可能为通过在牙周炎治疗药物（即牙周袋和组织贴片）中添加合适浓度的氟化钠来促进牙周再生提供一种新型治疗方法。
