Department of Biotechnology, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
ITC, Life Sciences and Technology Centre, Bengaluru, India.
Microbiol Res. 2021 Jan;242:126601. doi: 10.1016/j.micres.2020.126601. Epub 2020 Sep 25.
Plants are boon to the mankind due to plenty of metabolites with medicinal values. Though plants have traditionally been used to treat various diseases, their biological values are not completely explored yet. Sapindus mukorossi is one such ethnobotanical plant identified for various biological activities. As biofilm formation and biofilm mediated drug resistance of methicillin-resistant Staphylococcus aureus (MRSA) have raised as serious global issue, search for antibiofilm agents has gained greater importance. Notably, antibiofilm potential of S. mukorossi is still unexplored. The aim of the study is to explore the effect of S. mukorossi methanolic extract (SMME) on MRSA biofilm formation and adhesive molecules production. Significantly, SMME exhibited 82 % of biofilm inhibition at 250 μg/mL without affecting the growth and microscopic analyses evidenced the concentration dependent antibiofilm activity of SMME. In vitro assays exhibited the reduction in slime, cell surface hydrophobicity, autoaggregation, extracellular polysaccharides substance and extracellular DNA synthesis upon SMME treatment. Further, qPCR analysis confirmed the ability of SMME to interfere with the expression of adhesion genes associated with biofilm formation such as icaA, icaD, fnbA, fnbB, clfA, cna, and altA. GC-MS analysis and molecular docking study revealed that oleic acid is responsible for the antibiofilm activity. FT-IR analysis validated the presence of oleic acid in SMME. These results suggest that SMME can be used as a promising therapeutic agent against MRSA biofilm-associated infections.
由于具有药用价值的大量代谢物，植物是人类的福音。尽管植物传统上被用于治疗各种疾病，但它们的生物学价值尚未完全被探索。无患子是一种被鉴定具有多种生物活性的民族植物学植物。由于耐甲氧西林金黄色葡萄球菌（MRSA）的生物膜形成和生物膜介导的耐药性已成为严重的全球性问题，因此寻找抗生物膜剂变得更为重要。值得注意的是，无患子的抗生物膜潜力尚未得到探索。本研究旨在探索无患子甲醇提取物（SMME）对 MRSA 生物膜形成和黏附分子产生的影响。值得注意的是，SMME 在 250μg/mL 时表现出 82%的生物膜抑制作用，而不影响生长，并且显微镜分析证明了 SMME 的浓度依赖性抗生物膜活性。体外试验表明，在用 SMME 处理后，菌体内的黏液、细胞表面疏水性、自动聚集、细胞外多糖物质和细胞外 DNA 合成减少。此外，qPCR 分析证实了 SMME 能够干扰与生物膜形成相关的黏附基因的表达，如 icaA、icaD、fnbA、fnbB、clfA、cna 和 altA。GC-MS 分析和分子对接研究表明，油酸是抗生物膜活性的原因。FT-IR 分析验证了 SMME 中存在油酸。这些结果表明，SMME 可作为治疗耐甲氧西林金黄色葡萄球菌生物膜相关感染的有前途的治疗剂。
