Department of Applied Chemistry, University of Yamanashi.
Shinwa Chemical Industries Ltd.
Anal Sci. 2021 Feb 10;37(2):341-345. doi: 10.2116/analsci.20P311. Epub 2020 Oct 2.
Ifosfamide (IF), cyclophosphamide (CP), and bendamustine (BD) are widely used anticancer drugs. These drugs have slight volatility; therefore, medical-staff exposure is of concern in the medical field. However, an accurate and quantitative detection method of these volatile drugs in air has not been reported. In this study, we developed the quantitative extraction and detection method of these volatile anticancer drugs in air. For the extraction of analytes, a solid-phase extraction-type collection device packed with styrene-divinylbenzene polymer particles was used. The extracted analytes were quantitatively eluted with 5 mL of ethanol, and the solution was concentrated to 100 μL with nitrogen purging. The analytes were analyzed using gas chromatography-mass spectrometry (GC-MS). The limit of detection of the proposed method for IF and CP was 0.017 and 0.033 ng L, respectively in air at an air sampling volume of 300 L. IF and CP showed slight volatility, whereas BD was not detected in GC-MS due to its lower volatility. The spiked recoveries of IF and CP in the proposed method were within the range of 95.5 to 101%. Finally, the proposed method was applied to determine the exposure of IF and CP during the dispensing of CP within a hospital dispensary room. The investigated volatile anticancer drugs were not detected in real air samples, indicating that the protection measures employed are sufficient.
异环磷酰胺（IF）、环磷酰胺（CP）和苯达莫司汀（BD）是广泛使用的抗癌药物。这些药物具有轻微的挥发性；因此，在医疗领域，医务人员的接触受到关注。然而，尚未报道这些挥发性药物在空气中的准确和定量检测方法。在这项研究中，我们开发了一种定量提取和检测空气中这些挥发性抗癌药物的方法。对于分析物的提取，使用填充有苯乙烯-二乙烯基苯聚合物颗粒的固相萃取型收集装置。用 5 毫升乙醇定量洗脱提取的分析物，并用氮气吹扫将溶液浓缩至 100 微升。使用气相色谱-质谱联用仪（GC-MS）分析分析物。在空气采样体积为 300 升的情况下，该方法对 IF 和 CP 的检测限分别为空气中的 0.017 和 0.033ng/L。IF 和 CP 具有轻微的挥发性，而由于其挥发性较低，BD 在 GC-MS 中未被检测到。在该方法中，IF 和 CP 的加标回收率在 95.5%至 101%范围内。最后，该方法应用于测定医院配药室中 CP 配药过程中 IF 和 CP 的暴露情况。在实际空气样本中未检测到研究的挥发性抗癌药物，表明所采用的防护措施是充分的。
