小鼠脑内胶质母细胞瘤发展的纵向活体光学相干断层扫描成像
Longitudinal in-vivo OCM imaging of glioblastoma development in the mouse brain.
作者信息
Dolezyczek Hubert, Rapolu Mounika, Niedzwiedziuk Paulina, Karnowski Karol, Borycki Dawid, Dzwonek Joanna, Wilczynski Grzegorz, Malinowska Monika, Wojtkowski Maciej
机构信息
Nencki Institute of Experimental Biology, Polish Academy of Sciences, ul. Pasteura 3, 02-093 Warsaw, Poland.
both authors contributed equally.
出版信息
Biomed Opt Express. 2020 Aug 12;11(9):5003-5016. doi: 10.1364/BOE.400723. eCollection 2020 Sep 1.
We present in-vivo imaging of the mouse brain using custom made Gaussian beam optical coherence microscopy (OCM) with 800nm wavelength. We applied new instrumentation to longitudinal imaging of the glioblastoma (GBM) tumor microvasculature in the mouse brain. We have introduced new morphometric biomarkers that enable quantitative analysis of the development of GBM. We confirmed quantitatively an intensive angiogenesis in the tumor area between 3 and 14 days after GBM cells injection confirmed by considerably increased of morphometric parameters. Moreover, the OCM setup revealed heterogeneity and abnormality of newly formed vessels.
我们使用定制的波长为800nm的高斯光束光学相干显微镜（OCM）对小鼠大脑进行了体内成像。我们将新仪器应用于小鼠大脑中胶质母细胞瘤（GBM）肿瘤微血管系统的纵向成像。我们引入了新的形态计量生物标志物，能够对GBM的发展进行定量分析。通过形态计量参数的显著增加，我们定量证实了GBM细胞注射后3至14天肿瘤区域内强烈的血管生成。此外，OCM装置揭示了新形成血管的异质性和异常性。
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