Chu Liliang, Wang Shaowei, Li Kanghui, Xi Wang, Zhao Xinyuan, Qian Jun
State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang University; Zhejiang Provincial Key Laboratory for Sensing Technologies; Joint Research Center of Photonics of the Royal Institute of Technology (Sweden) and Zhejiang University, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
Department of Neurobiology, School of Medicine, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Biomed Opt Express. 2014 Oct 28;5(11):4076-88. doi: 10.1364/BOE.5.004076. eCollection 2014 Nov 1.
Near-infrared (NIR) imaging technology has been widely used for biomedical research and applications, since it can achieve deep penetration in biological tissues due to less absorption and scattering of NIR light. In our research, polymer nanoparticles with NIR fluorophores doped were synthesized. The morphology, absorption/emission features and chemical stability of the fluorescent nanoparticles were characterized, separately. NIR fluorescent nanoparticles were then utilized as bright optical probes for macro in vivo imaging of mice, including sentinel lymph node (SLN) mapping, as well as distribution and excretion monitoring of nanoparticles in animal body. Furthermore, we applied the NIR fluorescent nanoparticles in in vivo microscopic bioimaging via a confocal microscope. Under the 635 nm-CW excitation, the blood vessel architecture in the ear and the brain of mice, which were administered with nanoparticles, was visualized very clearly. The imaging depth of our one-photon microscopy, which was assisted with NIR fluorescent nanoprobes, can reach as deep as 500 μm. Our experiments show that NIR fluorescent nanoparticles have great potentials in various deep-tissue imaging applications.
近红外（NIR）成像技术已广泛应用于生物医学研究和应用领域，因为近红外光的吸收和散射较少，所以它可以在生物组织中实现深度穿透。在我们的研究中，合成了掺杂近红外荧光团的聚合物纳米颗粒。分别对荧光纳米颗粒的形态、吸收/发射特性和化学稳定性进行了表征。然后，将近红外荧光纳米颗粒用作明亮的光学探针，用于小鼠体内宏观成像，包括前哨淋巴结（SLN）定位，以及纳米颗粒在动物体内的分布和排泄监测。此外，我们通过共聚焦显微镜将近红外荧光纳米颗粒应用于体内微观生物成像。在635 nm连续波激发下，注射了纳米颗粒的小鼠耳朵和大脑中的血管结构清晰可见。我们的单光子显微镜在近红外荧光纳米探针的辅助下，成像深度可达500μm。我们的实验表明，近红外荧光纳米颗粒在各种深部组织成像应用中具有巨大潜力。
