Department of Clinical Laboratory, The Tumor Hospital of Guizhou Province, Guiyang, 550004 Guizhou, China.
Clinical Laboratory Medicine, Guizhou Medical University, Guiyang, 550004 Guizhou, China.
Biomed Res Int. 2020 Sep 16;2020:2474235. doi: 10.1155/2020/2474235. eCollection 2020.
The aim of this study was to observe the expression of miR-9, miR-21, miR-27b, and miR-34a related with E6/E7 in HPV16-, HPV52-, and HPV58-infected cervical cancer patients and explore their possible role in cervical cancer with HPV infection. The expression levels of 4 miRNAs were detected in cervical exfoliated cells using qRT-PCR. In the current study, miR-34a expression was significantly upregulated in HPV-positive cervical cancer compared with the HPV-negative healthy population and HPV-positive CIN, but just the expression of miR-34a in HPV16 cervical cancer was statistically significant, and the expression of HPV52 and HPV58 was not statistically significant. The expression of miR-21 increased in HPV-positive cervical cancer compared with HPV-positive CIN, but only HPV16-infected cervical cancer had statistical significance compared with HPV16-infected CIN. By observing the change trend of each subtype group, we can show that the expression of miR-9 in HPV16 CIN was opposite to the other subtypes, and it was upregulated, compared with HPV58 CIN, and significantly increased. The level change of miR-27b in HPV58 cervical cancer and HPV58 CIN was opposite to the other subtypes; unlike the expression of miR-27b which was upregulated in HPV16 and HPV52 infected, it was downregulated compared with Normal. We also found that the expression of miR-34a and miR-9 was contrary to other studies. These findings indicate that the upregulated miR-21 expression may be a biomarker to distinguish between CC and CIN. miR-34a in HPV infection, especially in HPV16 infection, might be related to the occurrence and development of cervical cancer. The infection of different subtypes may play different roles in disease by activating different mechanisms; miRNAs play a very complex role in tumorigenesis and development, and there may be multiple targets in which multiple mechanisms play a role.
本研究旨在观察 HPV16、HPV52 和 HPV58 感染的宫颈癌患者中与 E6/E7 相关的 miR-9、miR-21、miR-27b 和 miR-34a 的表达，并探讨它们在 HPV 感染相关宫颈癌中的可能作用。采用 qRT-PCR 法检测宫颈脱落细胞中 4 种 miRNA 的表达水平。本研究中，HPV 阳性宫颈癌患者 miR-34a 的表达明显高于 HPV 阴性健康人群和 HPV 阳性 CIN，但只有 HPV16 宫颈癌 miR-34a 的表达具有统计学意义，而 HPV52 和 HPV58 的表达无统计学意义。HPV 阳性宫颈癌患者 miR-21 的表达高于 HPV 阳性 CIN，但只有 HPV16 感染的宫颈癌与 HPV16 感染的 CIN 相比具有统计学意义。通过观察各亚型组的变化趋势，我们可以发现 HPV16 型 CIN 中 miR-9 的表达与其他亚型相反，与 HPV58 型 CIN 相比上调，且差异有统计学意义。HPV58 型宫颈癌和 HPV58 型 CIN 中 miR-27b 的水平变化与其他亚型相反；与 HPV16 和 HPV52 感染时 miR-27b 上调不同，其表达水平较正常下调。我们还发现 miR-34a 和 miR-9 的表达与其他研究相反。这些发现表明，上调的 miR-21 表达可能是区分宫颈癌和 CIN 的生物标志物。HPV 感染，特别是 HPV16 感染，miR-34a 可能与宫颈癌的发生发展有关。不同亚型的感染可能通过激活不同的机制在疾病中发挥不同的作用；miRNAs 在肿瘤的发生和发展中起着非常复杂的作用，可能存在多个靶点，多个机制发挥作用。
